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PXD021601

PXD021601 is an original dataset announced via ProteomeXchange.

Dataset Summary
TitleRegulatory function of sympathetic innervation on the endo/lysosomal trafficking of acetylcholine receptor
DescriptionRegulation of membrane receptors involves management of endocytosis. At the neuromuscular junction, the synapse between skeletal muscle and motoneuron, proper density of the major receptor, the acetylcholine receptor, is of utmost importance for sustaining life in context of mobility. Recent work has revealed innervation of NMJs by sympathetic neurons and destruction of them had morphological and functional consequences, suggesting influence on endocytosis. To investigate the pathways and proteins that are relevant for acetylcholine receptor turnover and affected by sympathetic signaling, proteomes of mouse hindlimb muscles from sympathectomized and saline-treated control muscles were compared. Using proteomic, Western blot, and immunofluorescence analysis in chemically sympathectomized mouse hindlimb muscles, the cause of these consequences were aimed to analyzed. This revealed extensive regulation of the proteome by the sympathetic nervous system and a possible regulatory function of the endo/lysosomal and autophagic pathway by sympathetic neuronal input. This finding might provide a new explanation to the observed benefit of sympathicomimetic treatment in several congenital myasthenic syndromes.
HostingRepositoryPRIDE
AnnounceDate2021-03-01
AnnouncementXMLSubmission_2021-03-01_02:23:13.440.xml
DigitalObjectIdentifier
ReviewLevelPeer-reviewed dataset
DatasetOriginOriginal dataset
RepositorySupportUnsupported dataset by repository
PrimarySubmitterLaxmikanth Kollipara
SpeciesList scientific name: Mus musculus (Mouse); NCBI TaxID: 10090;
ModificationListiTRAQ8plex-116 reporter+balance reagent acylated residue; monohydroxylated residue; iodoacetamide derivatized residue
InstrumentQ Exactive
Dataset History
RevisionDatetimeStatusChangeLog Entry
02020-09-21 23:11:51ID requested
12021-03-01 02:23:13announced
Publication List
Dataset with its publication pending
Keyword List
submitter keyword: sympathectomy, sympathetic nervous system, neuromuscular junction, skeletal muscle, endo/lysosomal trafficking, acetylcholine receptor
Contact List
Prof. Dr. Albert Sickmann
contact affiliationLeibniz-Institut für Analytische Wissenschaften – ISAS – e.V. Otto-Hahn-Straße 6b 44227 Dortmund, Germany P: +49 (0)2 31.13 92-100 F: +49 (0)2 31.13 92-200 www.isas.de
contact emailsickmann@isas.de
lab head
Laxmikanth Kollipara
contact affiliationLeibniz – Institut für Analytische Wissenschaften - ISAS - e.V.
contact emaillaxmikanth.kollipara@isas.de
dataset submitter
Full Dataset Link List
Dataset FTP location
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