PXD021601 is an
original dataset announced via ProteomeXchange.
Dataset Summary
Title | Regulatory function of sympathetic innervation on the endo/lysosomal trafficking of acetylcholine receptor |
Description | Regulation of membrane receptors involves management of endocytosis. At the neuromuscular junction, the synapse between skeletal muscle and motoneuron, proper density of the major receptor, the acetylcholine receptor, is of utmost importance for sustaining life in context of mobility. Recent work has revealed innervation of NMJs by sympathetic neurons and destruction of them had morphological and functional consequences, suggesting influence on endocytosis. To investigate the pathways and proteins that are relevant for acetylcholine receptor turnover and affected by sympathetic signaling, proteomes of mouse hindlimb muscles from sympathectomized and saline-treated control muscles were compared. Using proteomic, Western blot, and immunofluorescence analysis in chemically sympathectomized mouse hindlimb muscles, the cause of these consequences were aimed to analyzed. This revealed extensive regulation of the proteome by the sympathetic nervous system and a possible regulatory function of the endo/lysosomal and autophagic pathway by sympathetic neuronal input. This finding might provide a new explanation to the observed benefit of sympathicomimetic treatment in several congenital myasthenic syndromes. |
HostingRepository | PRIDE |
AnnounceDate | 2021-03-01 |
AnnouncementXML | Submission_2021-03-01_02:23:13.440.xml |
DigitalObjectIdentifier | |
ReviewLevel | Peer-reviewed dataset |
DatasetOrigin | Original dataset |
RepositorySupport | Unsupported dataset by repository |
PrimarySubmitter | Laxmikanth Kollipara |
SpeciesList | scientific name: Mus musculus (Mouse); NCBI TaxID: 10090; |
ModificationList | iTRAQ8plex-116 reporter+balance reagent acylated residue; monohydroxylated residue; iodoacetamide derivatized residue |
Instrument | Q Exactive |
Dataset History
Revision | Datetime | Status | ChangeLog Entry |
0 | 2020-09-21 23:11:51 | ID requested | |
⏵ 1 | 2021-03-01 02:23:13 | announced | |
Publication List
Dataset with its publication pending |
Keyword List
submitter keyword: sympathectomy, sympathetic nervous system, neuromuscular junction, skeletal muscle, endo/lysosomal trafficking, acetylcholine receptor |
Contact List
Prof. Dr. Albert Sickmann |
contact affiliation | Leibniz-Institut für Analytische Wissenschaften – ISAS – e.V. Otto-Hahn-Straße 6b 44227 Dortmund, Germany P: +49 (0)2 31.13 92-100 F: +49 (0)2 31.13 92-200 www.isas.de |
contact email | sickmann@isas.de |
lab head | |
Laxmikanth Kollipara |
contact affiliation | Leibniz – Institut für Analytische Wissenschaften - ISAS - e.V. |
contact email | laxmikanth.kollipara@isas.de |
dataset submitter | |
Full Dataset Link List
Dataset FTP location
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PRIDE project URI |
Repository Record List
[ + ]
[ - ]
- PRIDE
- PXD021601
- Label: PRIDE project
- Name: Regulatory function of sympathetic innervation on the endo/lysosomal trafficking of acetylcholine receptor