PXD021543

PXD021543 is an original dataset announced via ProteomeXchange.

Title	Flavonol-mediated stabilization of efflux complexes regulates polar auxin transport
Description	The transport of auxin controls the rate, direction and localization of plant growth and development. The course of auxin transport is defined by the polar subcellular localization of the PIN proteins, a family of auxin efflux transporters. Using blue native PAGE and quantitative mass spectrometry, we identify native PIN core transport units as homo- and heteromers assembled from PIN1, 2, 3, 4 and 7 subunits only. To identify the protein composition of the larger, low stringency-solubilized PIN assembly we affinity-purified GFP-fused PIN proteins from Arabidopsis roots after expression from their native promoters and after the induction of discrete lateral roots, and analyzed the captured proteins by label-free quantitative mass spectrometry (LC-MS/MS).
HostingRepository	PRIDE
AnnounceDate	2020-10-08
AnnouncementXML	Submission_2020-10-07_23:00:54.xml
DigitalObjectIdentifier	https://dx.doi.org/10.6019/PXD021543
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Supported dataset by repository
PrimarySubmitter	Alexander Haupt
SpeciesList	scientific name: Arabidopsis thaliana (Mouse-ear cress); NCBI TaxID: 3702;
ModificationList	S-carboxamidoethyl-L-cysteine; 2-pyrrolidone-5-carboxylic acid (Glu); 2-pyrrolidone-5-carboxylic acid (Gln); phosphorylated residue; acetylated residue; monohydroxylated residue; iodoacetamide derivatized residue
Instrument	LTQ Orbitrap; LTQ Orbitrap Elite

Revision	Datetime	Status	ChangeLog Entry
0	2020-09-17 08:37:06	ID requested
⏵ 1	2020-10-07 23:00:55	announced

Dataset with its publication pending

submitter keyword: Arabidopsis thaliana, auxin transport, AP-MS

Prof. Dr. Bernd Fakler
contact affiliation	Institute of Physiology II Universität Freiburg Germany
contact email	bernd.fakler@physiologie.uni-freiburg.de
lab head
Alexander Haupt
contact affiliation	University of Freiburg, Germany
contact email	alexander.haupt@physiologie.uni-freiburg.de
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2020/10/PXD021543

PRIDE project URI

• PRIDE
  1. PXD021543
     1. Label: PRIDE project
     2. Name: Flavonol-mediated stabilization of efflux complexes regulates polar auxin transport