PXD021538 is an
original dataset announced via ProteomeXchange.
Dataset Summary
Title | Top-down proteomics of human saliva in Alzheimer disease |
Description | Alzheimer disease (AD) is the most prevalent neurodegenerative disease and form of dementia in the elderly. It is characterized by the accumulation in the brain of misfolded proteins, inflammation and oxidative damage leading to region-specific loss of synaptic contacts and neuronal cell death. In the present study, we aimed to characterize the salivary protein profile of a sample (sample group or cohort) of AD patients in comparison with healthy controls (HC) by a top-down proteomic approach, to evidence possible quali-/quantitative variations associated to the disease. This approach is based on high performance liquid chromatography separation coupled to electrospray-ion trap mass spectrometry (HPLC-ESI-IT-MS) analysis of the acidic soluble proteome of human saliva, which was standardized in our previous studies for detecting and quantifying of hundreds salivary peptides/proteins. By this, it is possible to obtain a profiling of the naturally occurring salivary proteome, including isoforms and post-translational modifications (PTMs), to compare an unlimited number of samples and to quantify proteins by a label-free method. |
HostingRepository | PRIDE |
AnnounceDate | 2021-05-24 |
AnnouncementXML | Submission_2021-05-24_04:11:37.237.xml |
DigitalObjectIdentifier | |
ReviewLevel | Peer-reviewed dataset |
DatasetOrigin | Original dataset |
RepositorySupport | Unsupported dataset by repository |
PrimarySubmitter | Simone Serrao |
SpeciesList | scientific name: Homo sapiens (Human); NCBI TaxID: 9606; |
ModificationList | 2-pyrrolidone-5-carboxylic acid (Gln); S-nitrosyl-L-cysteine; monoacetylated residue; phosphorylated residue; disulfide crosslinked residues; monohydroxylated residue; O-phospho-L-threonine with neutral loss of phosphate; L-cysteine glutathione disulfide |
Instrument | Q Exactive HF |
Dataset History
Revision | Datetime | Status | ChangeLog Entry |
0 | 2020-09-17 08:36:50 | ID requested | |
⏵ 1 | 2021-05-24 04:11:37 | announced | |
Publication List
Dataset with its publication pending |
Keyword List
submitter keyword: Alzheimer disease, salivary proteomics, S100A, cystatins, α-defensins, thymosin β4, antimicrobial peptides, oxidative stress |
Contact List
Tiziana Cabras |
contact affiliation | Life and Environmental Sciences Department (Biomedical Section) - University of Cagliari |
contact email | tcabras@unica.it |
lab head | |
Simone Serrao |
contact affiliation | Life and Environmental Sciences Department (Biomedical Section) - University of Cagliari |
contact email | simone.serrao@unica.it |
dataset submitter | |
Full Dataset Link List
Dataset FTP location
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PRIDE project URI |
Repository Record List
[ + ]
[ - ]
- PRIDE
- PXD021538
- Label: PRIDE project
- Name: Top-down proteomics of human saliva in Alzheimer disease