PXD021521
PXD021521 is an original dataset announced via ProteomeXchange.
Dataset Summary
| Title | In-depth analysis of proteomic and genomic fluctuations during the time course of human embryonic stem cells directed differentiation into beta cells |
| Description | Pluripotent stem cells (PSC) endocrine differentiation at a large scale allows sampling of transcriptome and proteome with phosphoproteome (proteoform) at specific time points. We describe the dynamic time course of changes in cells undergoing directed beta cell differentiation and show target proteins or previously unknown phosphorylation of critical proteins in pancreas development, NKX6.1, and Chromogranin A (CHGA). We describe fluctuations in the correlation between gene expression, its protein abundance, and phosphorylation, which follow differentiation protocol perturbations of cell fates at all stages to id entify proteoform profiles. Our computational modeling recognizes outliers on a phenomic landscape of endocrine differentiation, and we outline several new biological pathways involved. We also suggest that non correlating proteins abundances or new phosphorylation motifs of NKX6.1 and CHGA point to new signaling pathways that may play an essential role in beta cell development. |
| HostingRepository | MassIVE |
| AnnounceDate | 2021-10-18 |
| AnnouncementXML | Submission_2021-10-18_09:17:33.970.xml |
| DigitalObjectIdentifier | |
| ReviewLevel | Non peer-reviewed dataset |
| DatasetOrigin | Original dataset |
| RepositorySupport | Supported dataset by repository |
| PrimarySubmitter | Bogdan Budnik |
| SpeciesList | scientific name: Homo sapiens; common name: human; NCBI TaxID: 9606; |
| ModificationList | Oxidation; Deamidated; TMT6plex; Carbamidomethyl |
| Instrument | Orbitrap Fusion Lumos |
Dataset History
| Revision | Datetime | Status | ChangeLog Entry |
|---|---|---|---|
| 0 | 2020-09-16 11:43:01 | ID requested | |
| ⏵ 1 | 2021-10-18 09:17:34 | announced |
Publication List
| no publication |
Keyword List
| submitter keyword: Pluripotent stem cells, beta cell differentiation, proteogenomics, phosphoproteomics |
Contact List
| Dmitry Shvartsman | |
|---|---|
| contact affiliation | Harvard University |
| contact email | dshvartsman@cellariabio.com |
| lab head | |
| Bogdan Budnik | |
| contact affiliation | Harvard University |
| contact email | bbudnik@mcb.harvard.edu |
| dataset submitter | |
Full Dataset Link List
| MassIVE dataset URI |
| Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://massive.ucsd.edu/MSV000086154/ |
to receive all new ProteomeXchange dataset release announcements!
