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PXD021371

PXD021371 is an original dataset announced via ProteomeXchange.

Dataset Summary
TitleDCLK1 small extracellular vesicle proteome
DescriptionDoublecortin-like kinase 1 (DCLK1) is a putative cancer stem cell marker, a promising diagnostic and prognostic maker for malignant tumors and a proposed driver gene for gastric cancer. Here we show that DCLK1 expression in the gastric cancer cell line MKN1 impacts EV biogenesis both quantitatively and qualitatively in a kinase dependent manner, revealing a hitherto unknown role for this putative oncogenic kinase. Importantly, the proteome of EVs isolated from DCLK1 expressing gastric cancer cells is enriched for proteins involved in tumor-promoting biological processes, raising the prospect of establishing DCLK1 as a novel anti-cancer target.
HostingRepositoryPRIDE
AnnounceDate2021-03-23
AnnouncementXMLSubmission_2021-03-22_23:10:57.792.xml
DigitalObjectIdentifier
ReviewLevelPeer-reviewed dataset
DatasetOriginOriginal dataset
RepositorySupportUnsupported dataset by repository
PrimarySubmitterdavid greening
SpeciesList scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationListacetylated residue; iodoacetamide derivatized residue
InstrumentQ Exactive HF
Dataset History
RevisionDatetimeStatusChangeLog Entry
02020-09-09 01:09:50ID requested
12021-03-22 23:10:58announced
Publication List
Dataset with its publication pending
Keyword List
submitter keyword: DCLK1, proteomics, exosomes, extracellular vesicles, migration
Contact List
David Greening
contact affiliationBaker Institute
contact emaildavid.greening@baker.edu.au
lab head
david greening
contact affiliationBaker Heart and Diabetes Inst.
contact emaildavid.greening@baker.edu.au
dataset submitter
Full Dataset Link List
Dataset FTP location
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PRIDE project URI
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