PXD021366 is an
original dataset announced via ProteomeXchange.
Dataset Summary
Title | Phosphoproteome profiling uncovers a key role for CDKs in TNF signaling |
Description | Tumor necrosis factor (TNF) is one of the few cytokines successfully targeted by therapies against inflammatory diseases. However, blocking this well studied and pleiotropic ligand can cause dramatic side-effects. We reasoned that a systems-level proteomic analysis of TNF signaling could dissect its diverse functions and offer a base for developing more targeted therapies. Combining phosphoproteomics time course experiments with spatial and kinase inhibitor analysis clusters phosphorylations into functional modules. The majority of regulated phosphorylations could be assigned to an upstream kinase by inhibiting master kinases and spatial proteomics revealed phosphorylation-dependent translocations of hundreds of proteins upon TNF stimulation. Phosphoproteome analysis of TNF-induced apoptosis and necroptosis revealed a key role for transcriptional cyclin-dependent kinase (CDK) activity to promote cytokine production and prevent excessive cell death downstream of the TNF signaling receptor. Our comprehensive interrogation of TNF induced pathways and sites can be explored at http://tnfviewer.biochem.mpg.de/. |
HostingRepository | PRIDE |
AnnounceDate | 2021-10-11 |
AnnouncementXML | Submission_2021-10-11_04:30:05.473.xml |
DigitalObjectIdentifier | |
ReviewLevel | Peer-reviewed dataset |
DatasetOrigin | Original dataset |
RepositorySupport | Unsupported dataset by repository |
PrimarySubmitter | Mario Oroshi |
SpeciesList | scientific name: Mus musculus (Mouse); NCBI TaxID: 10090; scientific name: Homo sapiens (Human); NCBI TaxID: 9606; |
ModificationList | phosphorylated residue |
Instrument | Q Exactive |
Dataset History
Revision | Datetime | Status | ChangeLog Entry |
0 | 2020-09-09 01:03:28 | ID requested | |
⏵ 1 | 2021-10-11 04:30:05 | announced | |
Publication List
Dataset with its publication pending |
Keyword List
submitter keyword: TNF, phosphoproteomics, cell death, spatial proteomics, apoptosis, necroptosis, Cyclin-dependent kinases, transcription, spatial proteomics, kinases |
Contact List
Matthias Mann |
contact affiliation | Department of Proteomics and Signal Transduction Max Planck Institute of Biochemistry Germany |
contact email | mmann@biochem.mpg.de |
lab head | |
Mario Oroshi |
contact affiliation | Proteomics |
contact email | oroshi@biochem.mpg.de |
dataset submitter | |
Full Dataset Link List
Dataset FTP location
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PRIDE project URI |
Repository Record List
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[ - ]
- PRIDE
- PXD021366
- Label: PRIDE project
- Name: Phosphoproteome profiling uncovers a key role for CDKs in TNF signaling