PXD021276
PXD021276 is an original dataset announced via ProteomeXchange.
Dataset Summary
Title | eEF2K inhibition synergizes with Glutaminase and eIF4EBP1 inhibitors to suppress triple-negative breast cancer cell growth; impact on the proteome |
Description | The eukaryotic elongation factor-2 kinase, eEF2K, restricts protein translation elongation, and was identified as a potential therapeutic target for diverse types of cancers including triple negative breast cancer (TNBC; PMID: 25330770). We have recently found that inhibition of eEF2K synergizes with depletion of eukaryotic translation initiation factor 4E-binding protein 1 (eIF4EBP1; 4E-BP1), a suppressor of eukaryotic protein translation initiation factor 4E (eIF4E), leading to effective growth suppression of TNBC cells in culture. We performed LC-MS/MS analysis following depletion of eEF2K and/or 4EBP in a TNBC cell line, Hs578t (YoungJun Ju & Eldad Zacksenhaus, manuscript in revisions). Depletion of these factors had overlapping effects on the proteome with the highest impact on Collagen containing extracellular matrix (e.g. COL1A1). The complete LC-MS/MS data sets (control vehicle; eEF2K-depletion, 4EBP1-depletion; combined eEF2k plus 4EBP1-depletion) are provided herein. |
HostingRepository | MassIVE |
AnnounceDate | 2020-11-16 |
AnnouncementXML | Submission_2020-11-16_06:52:26.xml |
DigitalObjectIdentifier | |
ReviewLevel | Non peer-reviewed dataset |
DatasetOrigin | Original dataset |
RepositorySupport | Supported dataset by repository |
PrimarySubmitter | YoungJun Ju |
SpeciesList | scientific name: Homo sapiens; common name: human; NCBI TaxID: 9606; |
ModificationList | Oxidation; Carbamidomethyl; Acetyl; TMT6plex |
Instrument | Orbitrap Fusion Lumos |
Dataset History
Revision | Datetime | Status | ChangeLog Entry |
---|---|---|---|
0 | 2020-09-02 10:25:49 | ID requested | |
⏵ 1 | 2020-11-16 06:52:26 | announced |
Publication List
no publication |
Keyword List
submitter keyword: eEF2K, 4EBP1, translation initiation, translation elongation, cancer therapy, triple negative breast cancer |
Contact List
YoungJun Ju | |
---|---|
contact affiliation | UHN |
contact email | yju@lakeheadu.ca |
lab head | |
Eldad Zacksenhaus | |
contact affiliation | UHN |
contact email | eldad.zacksenhaus@utoronto.ca |
lab head | |
YoungJun Ju | |
contact affiliation | UHN |
contact email | yju@lakeheadu.ca |
dataset submitter |
Full Dataset Link List
MassIVE dataset URI |
Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://massive.ucsd.edu/MSV000086059/ |