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PXD021190

PXD021190 is an original dataset announced via ProteomeXchange.

Dataset Summary
TitleSeven Novel Genes Related to Cell Proliferation and Migration of VHL-Mutated Pheochromocytoma
DescriptionPheochromocytoma has attracted much attention as a kind of neuroendocrine tumor with the highest genetic correlation in all types of tumors. Detailedly, the frequency of germline mutation in pheochromocytoma is as high as about 40%, and the mutation frequency of von Hippel Lindau (VHL) is the highest in all pheochromocytoma genes. However, the effects of VHL mutation on proteome of pheochromocytoma have remained unexplored. In this study, we compared and analyzed the proteomics of VHL-deficient and VHL WT PC12 cell line. It is showed that expressional level of 434 proteins (fold change > 1.3, VHL shRNA/WT) was significantly changed by VHL deficiency. Furthermore, the functions of these proteins were predicted by gene ontology (GO) and Kyoto Protocol Encyclopedia of Genes and Genomes (KEGG). Of the 434 proteins, 83 proteins, involved in cell proliferation, cell cycle, cell migration, and so on, were interpreted their complex regulatory relationships using String database. More importantly, we discovered 7 novel critical genes whose expression were up-regulated due to VHL deficiency, including Connective Tissue Growth Factor (CTGF), Syndecan Binding Protein (SDCBP), Cysteine Rich Protein 61 (CYR61/CCN1), Collagen Type III Alpha 1 Chain (COL3A1), Collagen Type I Alpha 1 Chain (COL1A1), Collagen Type V Alpha 2 Chain (COL5A2) and Serpin Family E Member 1 (SERPINE1), which may be related to cell proliferation and migration in VHL-mutated pheochromocytoma tumor samples. These results indicate that deficiency of VHL would promote the development of pheochromocytoma by activating expression of genes associated with cell proliferation and migration.
HostingRepositoryPRIDE
AnnounceDate2021-03-11
AnnouncementXMLSubmission_2021-03-11_05:14:37.012.xml
DigitalObjectIdentifier
ReviewLevelPeer-reviewed dataset
DatasetOriginOriginal dataset
RepositorySupportUnsupported dataset by repository
PrimarySubmitterFeizhou Zhu
SpeciesList scientific name: Rattus norvegicus (Rat); NCBI TaxID: 10116;
ModificationListNo PTMs are included in the dataset
InstrumentQ Exactive Plus
Dataset History
RevisionDatetimeStatusChangeLog Entry
02020-08-28 05:01:41ID requested
12021-03-11 05:14:37announced
Publication List
Dataset with its publication pending
Keyword List
submitter keyword: Pheochromocytoma
proliferation
migration
proteomics
VHL
Contact List
Feizhou Zhu
contact affiliationDepartment of Biochemistry and Molecular Biology, School of Life Sciences, Central South University, No. 172, Tongzipo road, Yuelu District, Changsha 410013, Hunan Province, China
contact emailzhufeizhou@csu.edu.cn
lab head
Feizhou Zhu
contact affiliationDepartment of Biochemistry and Molecular Biology, School of Life Sciences, Central South University, Changsha
contact emailzhufeizhou@csu.edu.cn
dataset submitter
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