PXD021124

PXD021124 is an original dataset announced via ProteomeXchange.

Title	Identification of IgE reactive proteins from Aspergillus terreus using mass spectrometry.
Description	Aspergillus terreus has been described as an allergenic fungus in addition to causing infection to both human beings and plants. However, the allergens in this fungus are still unknown. The identification of allergens is essential to develop diagnostic and therapeutic avenues. Employing a proteomic approach, sixteen allergens were identified based on two-dimensional immuno-blotting with A. terreus susceptible patient sera. Amongst them, triosephosphate isomerase (Asp t 36), one of the dominant immunoglobulin E (IgE)-reactive proteins, was purified under native conditions. To characterize Asp t 36, the gene was cloned and expressed in E. coli. Phylogenetic analysis showed it to be highly conserved with close similarity to Dermatophagoides farinae. Four immuno-dominant epitopes were identified using synthetic peptides and mapped on the tertiary structure of Asp t 36. Among these, two were found to create a continuous surface patch on the 3D structure rendering it to be an IgE binding hotspot. Asp t 36 showed similar secondary structure and temperature sensitivity with other reported triosephosphate isomerase allergens. In-vivo studies using the murine model demonstrated rAsp t 36 able to stimulate airway inflammation, such as an influx of eosinophils, goblet cell hyperplasia, elevated serum immunoglobulins and induction of Th2 cytokines. Collectively, our results reveal the immunogenic property of Asp t 36, a major allergen from A. terreus for the first time in any fungi. This allergen could serve as a potent candidate for investigating component resolved diagnosis and immunotherapy.
HostingRepository	PRIDE
AnnounceDate	2020-10-20
AnnouncementXML	Submission_2020-10-20_00:54:34.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Bijoya Karmakar
SpeciesList	scientific name: Aspergillus terreus NIH2624; NCBI TaxID: 341663;
ModificationList	iodoacetamide derivatized residue
Instrument	autoflex

Revision	Datetime	Status	ChangeLog Entry
0	2020-08-26 00:04:40	ID requested
⏵ 1	2020-10-20 00:54:34	announced

Dataset with its publication pending

submitter keyword: Aspergillus terreus, allergen, triosephosphate isomerase, proteomics, chromatography, circular dichroism, epitope mapping, airway inflammation, immunoglobulin E (IgE), immunoglobulin G (IgG).

Bijoya Karmakar and Swati Gupta Bhattacharya
contact affiliation	Bijoya Karmakar, Division of Plant Biology, Bose Institute, Kolkata, India. Swati Gupta Bhattacharya, Professor, Division of Plant Biology Bose Institute 93/1 Acharya Prafulla Chandra Road, Kolkata 700009
contact email	swati@jcbose.ac.in
lab head
Bijoya Karmakar
contact affiliation	Senior Research Fellow
contact email	bijoyak5@gmail.com
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD021124
     1. Label: PRIDE project
     2. Name: Identification of IgE reactive proteins from Aspergillus terreus using mass spectrometry.