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PXD020954

PXD020954 is an original dataset announced via ProteomeXchange.

Dataset Summary
TitleChicken microbiome LC - MS / MS
DescriptionBacteroidaceae are common gut microbiota members in all warm-blooded animals. However, if Bacteroidaceae are to be used as probiotics, the species selected for different hosts should reflect the natural distribution. In this study, we therefore evaluated host adaptation of bacterial species belonging to the family Bacteroidaceae. B. dorei, B. uniformis, B. xylanisolvens, B. ovatus, B. clarus, B. thetaiotaomicron and B. vulgatus represented human-adapted species while B. gallinaceum, B. caecigallinarum, B. mediterraneensis, B. caecicola, M. massiliensis, B. plebeius and B. coprocola were commonly detected in chicken but not human gut microbiota. There were 29 genes which were present in all human-adapted Bacteroides but absent from the genomes of all chicken isolates and these included genes required for the pentose cycle, and glutamate or histidine metabolism. These genes were expressed during an in vitro competitive assay, in which human-adapted Bacteroides species overgrew the chicken adapted isolates. Not a single gene specific for the chicken-adapted species was found. Instead, chicken adapted species exhibited signs of frequent horizontal gene transfer, of KUP, linA and sugE genes in particular. The differences in host adaptation should be considered when the new generation of probiotics for humans or chickens is designed.
HostingRepositoryPRIDE
AnnounceDate2020-09-28
AnnouncementXMLSubmission_2020-09-27_23:58:25.xml
DigitalObjectIdentifier
ReviewLevelPeer-reviewed dataset
DatasetOriginOriginal dataset
RepositorySupportUnsupported dataset by repository
PrimarySubmitterZuzana Seidlerova
SpeciesList scientific name: Bacteroides sp. 1_1_11; NCBI TaxID: 469584;
ModificationListmonohydroxylated residue; iodoacetamide derivatized residue
InstrumentLTQ Velos
Dataset History
RevisionDatetimeStatusChangeLog Entry
02020-08-17 03:35:53ID requested
12020-09-27 23:58:25announced
Publication List
Dataset with its publication pending
Keyword List
submitter keyword: Microbiota, Microbiome, Chicken, Caecum, Bacteroides
Contact List
doc. RNDr. RYCHLÍK Ivan, Ph.D.
contact affiliationVeterinary Research Institute Department:MICROBIOLOGY AND ANTIMICROBIAL RESISTANCE Hudcova 296/70 621 00 Brno Czech Republic
contact emailrychlik@vri.cz
lab head
Zuzana Seidlerova
contact affiliationVeterinary Research Institute Hudcova 296/70 621 00 Brno Czech Republic
contact emailseidlerova@vri.cz
dataset submitter
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Dataset FTP location
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PRIDE project URI
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