PXD020859 is an
original dataset announced via ProteomeXchange.
Dataset Summary
Title | Cross-linking mass spectrometry uncovers protein interactions and functional assemblies in synaptic vesicle membranes |
Description | Synaptic vesicles are storage organelles for neurotransmitters in the pre-synaptic cytosol. When an action potential arrives at the pre-synaptic terminal, they fuse with the pre-synaptic membrane and neurotransmitters are released. In this project, we set out to study the protein interactions formed in synaptic vesicle membranes. For this, we first assessed the proteome of five independent vesicle preparations. Using chemical cross-linking, we then tackled the interactions of the major protein components. To gain further insights, we combined this approach with a chemical labelling strategy. Specific protein interactions were then studied after (i) treating the vesicles with Botulinum neurotoxin B, (ii) binding the soluble SNARE complex, and (iii) fusion of the vesicles with empty liposomes. Our findings reveal stable interaction modules in synaptic vesicles. |
HostingRepository | PRIDE |
AnnounceDate | 2020-12-09 |
AnnouncementXML | Submission_2020-12-09_00:26:30.xml |
DigitalObjectIdentifier | |
ReviewLevel | Peer-reviewed dataset |
DatasetOrigin | Original dataset |
RepositorySupport | Unsupported dataset by repository |
PrimarySubmitter | Marie Barth |
SpeciesList | scientific name: Rattus norvegicus (Rat); NCBI TaxID: 10116; |
ModificationList | acetylated residue; monohydroxylated residue; iodoacetamide derivatized residue |
Instrument | Orbitrap Fusion; Q Exactive Plus |
Dataset History
Revision | Datetime | Status | ChangeLog Entry |
0 | 2020-08-11 17:03:58 | ID requested | |
⏵ 1 | 2020-12-09 00:26:30 | announced | |
Publication List
Dataset with its publication pending |
Keyword List
submitter keyword: Synaptic vesicles, protein networks, chemical cross-linking, mass spectrometry, Synaptobrevin-2, Synaptophysin |
Contact List
Carla Schmidt |
contact affiliation | Interdisciplinary Research Centre HALOmem, Charles Tanford Protein Centre, Institute for Biochemistry and Biotechnology, Martin Luther University Halle-Wittenberg, Halle, Germany |
contact email | carla.schmidt@biochemtech.uni-halle.de |
lab head | |
Marie Barth |
contact affiliation | HALOmem, Martin Luther University Halle-Wittenberg |
contact email | marie.barth@bct.uni-halle.de |
dataset submitter | |
Full Dataset Link List
Dataset FTP location
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PRIDE project URI |
Repository Record List
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- PRIDE
- PXD020859
- Label: PRIDE project
- Name: Cross-linking mass spectrometry uncovers protein interactions and functional assemblies in synaptic vesicle membranes