PXD020792

PXD020792 is an original dataset announced via ProteomeXchange.

Title	Trans-2-enoyl-CoA reductase limits Ca2+ accumulation in the endoplasmic reticulum by inhibiting SERCA2b Ca2+ pump
Description	The endoplasmic reticulum (ER) contains various enzymes that metabolize fatty acids (FAs). How FA metabolic enzymes cooperate with other ER functions, such as calcium (Ca2+) accumulation and its regulated release to the cytosol, is elusive. Trans-2-enoyl-CoA reductase (TER) is a FA reductase present in the ER membrane, and catalyzes the last step of FA elongation cycle and sphingosine degradation pathway. Here we show that TER directly binds to an ER Ca2+ pump, sarco(endo)plasmic reticulum Ca2+-ATPase 2b (SERCA2b), and regulates its activity. Thapsigargin, a specific SERCA inhibitor, inhibits this binding. TER binds to SERCA2b through its conserved C-terminal region. TER overexpression suppresses SERCA2b ATPase activity in microsomal membranes. Depletion of TER increases the ER Ca2+ storage and accelerates SERCA2b-dependent Ca2+ uptake to the ER after ligand-induced Ca2+ release. These results demonstrate that TER is a negative regulator of SERCA2b, implying the direct linkage of FA metabolism and Ca2+ accumulation in the ER.
HostingRepository	PRIDE
AnnounceDate	2021-01-13
AnnouncementXML	Submission_2021-01-13_01:25:26.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Toshiaki Sakisaka
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	monohydroxylated residue; iodoacetamide derivatized residue
Instrument	LCMS-IT-TOF

Revision	Datetime	Status	ChangeLog Entry
0	2020-08-07 04:20:02	ID requested
⏵ 1	2021-01-13 01:25:26	announced

Dataset with its publication pending

submitter keyword: fatty acid metabolism, endoplasmic reticulum, calcium, calcium ATPase, calcium transport, protein-protein interaction

Toshiaki Sakisaka
contact affiliation	Division of Membrane Dynamics, Department of Physiology and Cell Biology, Kobe University School of Medicine
contact email	sakisaka@med.kobe-u.ac.jp
lab head
Toshiaki Sakisaka
contact affiliation	Kobe University Graduate School of Medicine
contact email	sakisaka@med.kobe-u.ac.jp
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD020792
     1. Label: PRIDE project
     2. Name: Trans-2-enoyl-CoA reductase limits Ca2+ accumulation in the endoplasmic reticulum by inhibiting SERCA2b Ca2+ pump