PXD020788

PXD020788 is an original dataset announced via ProteomeXchange.

Title	Global analysis of lysine 2-hydroxyisobutyrylation in wheat leaf
Description	Lysine 2-hydroxyisobutyrylation (Khib) is a novel naturally occurring PTM, which is firstly discovered in histone. The system Khib detection at proteomics level has been performed in various species and tissues for the function and role characterization of Khib in biological activities. However, the Khib study in plant species is relatively less and the plant root, one of the critical plant organ, haven’t been studied. In the present study, the first root tissues lysine 2-hydroxyisobutyrylome analysis was performed in wheat specie with antibody immunoprecipitation affinity and high resolution mass spectrometry-based proteomics. Bioinformatics analyses including function classification, subcellular location predication, gene ontology enrichment and pathway enrichment were conducted to demonstrate the roles of these Khib sites and proteins in wheat root. In total, 6328 Khib sites corresponding to 2186 proteins were repeatedly identified in three replicates. These Khib proteins showed a wide subcellular location distribution while cytoplasm was the primary subcellular compartment where Khib protein located. Function and pathways characterization of these Khib proteins indicated that many cellular functions and metabolism pathways potentially were affected by this modification. Protein and amino acid metabolism related process may be regulated by Khib, especially ribosome activities and proteins biosynthesis process. Carbohydrate metabolism and energy production related processes including glycolysis/gluconeogenesis, TCA cycle and oxidative phosphorylation pathways were also the hotspots where protein Khib occurred. Our work illustrated the potential regulation role of Khib in wheat leaf physiology and biology, which could be used as a useful reference for Khib studies in plant leaf.
HostingRepository	PRIDE
AnnounceDate	2021-06-08
AnnouncementXML	Submission_2021-06-08_05:03:13.533.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Xianliang Ma
SpeciesList	scientific name: Triticum aestivum (Wheat); NCBI TaxID: 4565;
ModificationList	carbamoylated residue
Instrument	Q Exactive HF

Revision	Datetime	Status	ChangeLog Entry
0	2020-08-07 04:19:28	ID requested
⏵ 1	2021-06-08 05:03:13	announced

Dataset with its publication pending

submitter keyword: Key words

Lysine 2-hydroxyisobutyrylation, wheat leaf, post-translational modification, protein metabolism

Geng Li
contact affiliation	College of Agronomy, Shandong Agricultural University, Tai’an, China
contact email	ligeng213@sina.com
lab head
Xianliang Ma
contact affiliation	Micrometer Biotech
contact email	maxianliangyxz@163.com
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD020788
     1. Label: PRIDE project
     2. Name: Global analysis of lysine 2-hydroxyisobutyrylation in wheat leaf