PXD020736

PXD020736 is an original dataset announced via ProteomeXchange.

Dataset Summary

Title	The Capture of a Disabled Proteasome Identifies Erg25 as a Substrate for Endoplasmic Reticulum Associated Degradation
Description	Proteasome, proteasome partners and ubiquitin-proteasome system (UPS) substrates were affinity purified from a yeast strain with an GFP-tagged proteasome subunit to which a proteasome inhibitor could be applied. Parallel experiments utilized inhibitor insensitive strains or strains lacking the tagged subunit. After affinity isolation, enriched proteins were resolved in SDS-PAGE, in-gel digested, and analyzed by high resolution liquid chromatography-tandem MS.
HostingRepository	MassIVE
AnnounceDate	2020-09-10
AnnouncementXML	Submission_2020-09-10_08:43:29.xml
DigitalObjectIdentifier
ReviewLevel	Non peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Xuemei Zeng
SpeciesList	scientific name: Saccharomyces cerevisiae BY4741; NCBI TaxID: 1247190;
ModificationList	S-carboxamidomethyl-L-cysteine; L-methionine sulfoxide; deglycosylated asparagine; deamidated L-glutamine; ubiquitination signature dipeptidyl lysine
Instrument	LTQ Orbitrap Velos

Dataset History

Revision	Datetime	Status	ChangeLog Entry
0	2020-08-05 07:21:12	ID requested
⏵ 1	2020-09-10 08:43:29	announced

Publication List

no publication

no publication

Keyword List

submitter keyword: Proteasome, ubiquitin, yeast, proteomics, affinity purification, MG132

submitter keyword: Proteasome, ubiquitin, yeast, proteomics, affinity purification, MG132

Contact List

Jeffrey L. Brodsky
contact affiliation	University of Pittsburgh
contact email	jbrodsky@pitt.edu
lab head
Xuemei Zeng
contact affiliation	University of Pittsburgh
contact email	xuz2@pitt.edu
dataset submitter

Full Dataset Link List

MassIVE dataset URI
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MassIVE dataset URI

Dataset FTP location
NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://massive.ucsd.edu/MSV000085914/