PXD020731 is an
original dataset announced via ProteomeXchange.
Dataset Summary
Title | ErbB2 drives Yap activation during cardiac regeneration |
Description | Cardiomyocyte (CM) loss after injury results in adverse remodelling and fibrosis, which inevitably lead to heart failure. Neuregulin-ErbB2 and Hippo-Yap signaling pathways are key mediators of CM proliferation and regeneration although the crosstalk between these pathways is unclear. Here, we demonstrate in mice that temporal over-expression (OE) of activated ErbB2 in CMs promotes cardiac regeneration in a heart failure model. Cellularly, OE CMs present an EMT-like regenerative response involving cytoskeletal reprograming, migration, ECM turnover, and displacement. Molecularly, we identified Yap as a critical mediator of ErbB2 signaling. In OE CMs, Yap interacts with nuclear envelope and cytoskeletal components, reflective of the altered mechanic state elicited by ErbB2. Hippo-independent activating phosphorylation on Yap at S352 and S274 were enriched in OE CMs, peaking during metaphase. Viral overexpression of Yap phospho-mutants dampened the proliferative competence of OE CMs. Taken together, we demonstrate a potent ErbB2-mediated Yap mechanosensory signaling involving EMT-like characteristics, resulting in heart regeneration. |
HostingRepository | PRIDE |
AnnounceDate | 2020-08-21 |
AnnouncementXML | Submission_2020-08-21_06:17:39.xml |
DigitalObjectIdentifier | |
ReviewLevel | Peer-reviewed dataset |
DatasetOrigin | Original dataset |
RepositorySupport | Unsupported dataset by repository |
PrimarySubmitter | Alon Savidor |
SpeciesList | scientific name: Mus musculus (Mouse); NCBI TaxID: 10090; |
ModificationList | phosphorylated residue; monohydroxylated residue; deamidated residue |
Instrument | Q Exactive |
Dataset History
Revision | Datetime | Status | ChangeLog Entry |
0 | 2020-08-05 02:56:07 | ID requested | |
⏵ 1 | 2020-08-21 06:17:39 | announced | |
Publication List
Dataset with its publication pending |
Keyword List
submitter keyword: LFQ, DDA, phosphoproteomics, mouse, orbitrap, IP |
Contact List
Eldad Tzahor |
contact affiliation | Department of Molecular Cell Biology Weizmann Institute of Science |
contact email | eldad.tzahor@weizmann.ac.il |
lab head | |
Alon Savidor |
contact affiliation | The Weizmann Institute of Science |
contact email | alon.savidor@weizmann.ac.il |
dataset submitter | |
Full Dataset Link List
Dataset FTP location
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PRIDE project URI |
Repository Record List
[ + ]
[ - ]
- PRIDE
- PXD020731
- Label: PRIDE project
- Name: ErbB2 drives Yap activation during cardiac regeneration