PXD020481 is an
original dataset announced via ProteomeXchange.
Dataset Summary
Title | Light-mediated discovery of surfaceome nanoscale organization and intercellular receptor interaction networks |
Description | Delineating the extracellular interaction network of the cell surface proteotype (the surfaceome) is fundamental to understanding cellular signaling function in health and disease. Here, we developed LUX-MS, an optoproteomic technology that enables the spatiotemporal and proteome-wide study of surfaceome signaling architectures at nanoscale level without the need for genetic manipulation. A tunable, light-triggered singlet oxygen generator (SOG) based mechanism mediates in-situ proximity-tagging for subsequent mass spectrometry-based identification of acute protein interactions in their native cellular context. We applied LUX-MS to the characterization of surfaceome signaling structures engaged by antibodies, small molecule drugs, biologics and intact bacteriophages across organisms and in complex environments. Cell-type resolved dissection of intercellular communication using LUX-MS thereby revealed the molecular architecture of functional immunological signaling synapses in unprecedented detail. Altogether, LUX-MS enables facebooking of the social protein networks within surfaceome signaling architectures and provides an unprecedented molecular framework for the rational design of biomedical intervention strategies. |
HostingRepository | PRIDE |
AnnounceDate | 2021-06-14 |
AnnouncementXML | Submission_2021-06-14_15:22:33.309.xml |
DigitalObjectIdentifier | |
ReviewLevel | Peer-reviewed dataset |
DatasetOrigin | Original dataset |
RepositorySupport | Unsupported dataset by repository |
PrimarySubmitter | Maik Müller |
SpeciesList | scientific name: Mus musculus (Mouse); NCBI TaxID: 10090; scientific name: Escherichia coli; NCBI TaxID: 562; scientific name: Listeria monocytogenes serotype 4b; NCBI TaxID: 2065118; scientific name: Listeria phage A500; NCBI TaxID: 40522; scientific name: Homo sapiens (Human); NCBI TaxID: 9606; |
ModificationList | dihydroxylated residue; isotope labeled residue; deaminated residue; monohydroxylated residue |
Instrument | Orbitrap Fusion Lumos; Q Exactive HF; Orbitrap Fusion |
Dataset History
Revision | Datetime | Status | ChangeLog Entry |
0 | 2020-07-21 17:52:58 | ID requested | |
⏵ 1 | 2021-06-14 15:22:33 | announced | |
Publication List
Dataset with its publication pending |
Keyword List
submitter keyword: LC-MS/MS |
Contact List
Bernd Wollscheid |
contact affiliation | Institute of Translational Medicine, Department of Health Sciences and Technology, ETH Zurich, Zurich, Switzerland |
contact email | wbernd@ethz.ch |
lab head | |
Maik Müller |
contact affiliation | ETH Zurich |
contact email | maimuell@ethz.ch |
dataset submitter | |
Full Dataset Link List
Dataset FTP location
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PRIDE project URI |
Repository Record List
[ + ]
[ - ]
- PRIDE
- PXD020481
- Label: PRIDE project
- Name: Light-mediated discovery of surfaceome nanoscale organization and intercellular receptor interaction networks