PXD020355

PXD020355 is an original dataset announced via ProteomeXchange.

Title	Prothrombin is a binding partner of the human Receptor of Advanced Glycation End products
Description	The receptor for advanced glycation end products (RAGE) plays a key role in mammal physiology and in the etiology and progression of inflammatory and oxidative stress-based diseases. In adults, RAGE expression is high only in the lung where the protein concentrates in the basal membrane of Type I alveolar epithelial cells. In diseases, RAGE levels increase in the affected tissues and sustain chronic inflammation. RAGE exists as a single trans-membrane protein with an ectodomain and a short carboxyl-terminal tail essential for signaling, or also as a soluble ectodomain (sRAGE) that acts as a decoy receptor. The ectodomain is composed of a variable and two constant IgG-like domains (V, C1 and C2). VC1 is a single integrated unit responsible for binding of RAGE to the majority of its ligands including the advanced glycation End products (AGEs), S100 proteins and HMGB-1. Here, we report the identification of 20 new RAGE binding partners captured from human plasma and involved in the coagulation and complement processes and in the extracellular matrix. Four proteins contained a -carboxyl glutamic acid (Gla)-domain. Using MicroScale Thermophoresis, we quantified the interaction of prothrombin (PT) with VC1 and with sRAGE. Calcium competed in the binding of PT to sRAGE and PT devoid of the Gla-domain did not bind to sRAGE, providing evidence that the Gla-domain is critical for the interaction. Finally, the presence of VC1 delayed plasma clotting in a dose-dependent manner. We propose that RAGE is involved in modulating blood coagulation presumably in conditions of lung injury.
HostingRepository	PRIDE
AnnounceDate	2024-10-22
AnnouncementXML	Submission_2024-10-22_05:08:58.364.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Alessandra Altomare
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	monohydroxylated residue; iodoacetamide derivatized residue
Instrument	LTQ Orbitrap

Revision	Datetime	Status	ChangeLog Entry
0	2020-07-14 02:46:46	ID requested
1	2020-07-14 05:51:00	announced
⏵ 2	2024-10-22 05:08:58	announced	2024-10-22: Updated project metadata.

Dataset with its publication pending

submitter keyword: Receptor for advanced glycation end product (RAGE)

pattern recognition receptor (PRR)

scavenger receptor

prothrombin

coagulation factor

mass spectrometry (MS)

plasma

protein -carboxylation

lung injury

protein-protein interaction.

Alessandra Altomare
contact affiliation	University of Milan, DISFARM
contact email	alessandra.altomare@unimi.it
lab head
Alessandra Altomare
contact affiliation	University of Milan (DISFARM)
contact email	alessandra.altomare@unimi.it
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2020/07/PXD020355

PRIDE project URI

• PRIDE
  1. PXD020355
     1. Label: PRIDE project
     2. Name: Prothrombin is a binding partner of the human Receptor of Advanced Glycation End products