PXD020229

PXD020229 is an original dataset announced via ProteomeXchange.

Title	Crosslinking mass spectrometry of the unconventional kinetoplastid kinetochore protein KKT4
Description	The kinetochore is the macromolecular protein machine that drives chromosome segregation by interacting with spindle microtubules. Unlike most other eukaryotes that have canonical kinetochore proteins, a group of evolutionarily divergent eukaryotes called kinetoplastids (such as Trypanosoma brucei that causes human African trypanosomiasis) have a unique set of kinetochore proteins. To date, the only kinetoplastid kinetochore protein that is known to bind microtubules is KKT4, which has no high-resolution structure information available. Here we used X-ray crystallography, NMR spectroscopy, and crosslinking mass spectrometry to characterise the structure and dynamics of KKT4.
HostingRepository	PRIDE
AnnounceDate	2020-10-15
AnnouncementXML	Submission_2020-10-15_05:43:59.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Bungo Akiyoshi
SpeciesList	scientific name: Trypanosoma brucei; NCBI TaxID: 5691;
ModificationList	No PTMs are included in the dataset
Instrument	Q Exactive

Revision	Datetime	Status	ChangeLog Entry
0	2020-07-06 16:11:49	ID requested
⏵ 1	2020-10-15 05:43:59	announced

Dataset with its publication pending

submitter keyword: Kinetoplastid, kinetochore, KKT4, Trypanosoma brucei

Bungo Akiyoshi
contact affiliation	University of Oxford
contact email	bungo.akiyoshi@bioch.ox.ac.uk
lab head
Bungo Akiyoshi
contact affiliation	University of Oxford
contact email	bungo.akiyoshi@bioch.ox.ac.uk
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD020229
     1. Label: PRIDE project
     2. Name: Crosslinking mass spectrometry of the unconventional kinetoplastid kinetochore protein KKT4