PXD019953 is an
original dataset announced via ProteomeXchange.
Dataset Summary
Title | (p)ppGpp controls stringent factors by exploiting antagonistic allosteric coupling between catalytic domains |
Description | Escherichia coli RelA is a ribosomal factor with strong (p)ppGpp synthesis activity that is dramatically activated in the presence of deacylated tRNA in the ribosomal A-site. RelA is a unique enzyme in that it is directly positively regulated by its product, alarmone nucleotide (p)ppGpp. Using HDX-MS, mulecular docking, biochemsitry, and microbiology approaches, we localise the (p)ppGpp binding site of E. coli RelA and uncover the molecular mechanism of RelA regulation by the alarmone. |
HostingRepository | PRIDE |
AnnounceDate | 2024-10-22 |
AnnouncementXML | Submission_2024-10-22_05:24:34.374.xml |
DigitalObjectIdentifier | |
ReviewLevel | Peer-reviewed dataset |
DatasetOrigin | Original dataset |
RepositorySupport | Unsupported dataset by repository |
PrimarySubmitter | Simon Ekström |
SpeciesList | scientific name: Escherichia coli; NCBI TaxID: 562; |
ModificationList | No PTMs are included in the dataset |
Instrument | Q Exactive |
Dataset History
Revision | Datetime | Status | ChangeLog Entry |
0 | 2020-06-22 07:53:51 | ID requested | |
1 | 2021-08-11 05:28:46 | announced | |
⏵ 2 | 2024-10-22 05:24:34 | announced | 2024-10-22: Updated project metadata. |
Publication List
Dataset with its publication pending |
Keyword List
ProteomeXchange project tag: Hydrogen Deuterium Exchange (HDX-MS) |
submitter keyword: Escherichia coli / stringent response / RelA / (p)ppGpp |
Contact List
Vasili Hauryliuk |
contact affiliation | Department of Molecular Biology Umeå University SE-901 87 Umeå Sweden |
contact email | vasili.hauryliuk@umu.se |
lab head | |
Simon Ekström |
contact affiliation | BioMS - Swedish National Infrastructure for Biological Mass Spectrometry, Lund University, BMC D1330, 221 84 Lund, Sweden |
contact email | simon.ekstrom@med.lu.se |
dataset submitter | |
Full Dataset Link List
Dataset FTP location
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PRIDE project URI |
Repository Record List
[ + ]
[ - ]
- PRIDE
- PXD019953
- Label: PRIDE project
- Name: (p)ppGpp controls stringent factors by exploiting antagonistic allosteric coupling between catalytic domains