PXD019305 is an
original dataset announced via ProteomeXchange.
Dataset Summary
Title | Xylose Metabolism and the Effect of Oxidative Stress on Lipid and Carotenoid Production in Rhodotorula toruloides: Insights for Future Biorefinery |
Description | The use of cell factories to convert sugars from lignocellulosic biomass into chemicals in which oleochemicals and food additives, such as carotenoids, play an important role is essential for the shift towards sustainable processes. Rhodotorula toruloides is a yeast that naturally metabolises a wide range of substrates, including lignocellulosic hydrolysates, and converts them into lipids and carotenoids. In this study, xylose, the main component of hemicellulose, was used as the sole substrate for R. toruloides, and a detailed physiology characterisation combined with absolute proteomics and genome-scale metabolic models was carried out to understand the regulation of lipid and carotenoid production. To improve these productions, oxidative stress was induced by hydrogen peroxide and light irradiation and further enhanced by adaptive laboratory evolution. Based on the online measurements of growth and CO2 excretion, three distinct growth phases were identified during batch cultivations. The intracellular flux estimations correlated well with the measured protein levels and demonstrated improved NADPH regeneration and phosphoketolase activity and reduced beta-oxidation, correlating with increasing lipid yields. Light irradiation conditions resulted in 70% higher carotenoid and 40% higher lipid yields. The presence of hydrogen peroxide did not affect the carotenoid yield but culminated in the highest lipid yield of 0.65 ± 0.06 g/gDCW. The adapted strain showed improved fitness and 130% higher carotenoid yield than the parental strain. This work presented a holistic view of xylose conversion into microbial oil and carotenoids by R. toruloides for further cost-effective and renewable production of these molecules. |
HostingRepository | PRIDE |
AnnounceDate | 2020-07-29 |
AnnouncementXML | Submission_2020-07-29_04:20:28.xml |
DigitalObjectIdentifier | |
ReviewLevel | Peer-reviewed dataset |
DatasetOrigin | Original dataset |
RepositorySupport | Unsupported dataset by repository |
PrimarySubmitter | Petri-Jaan Lahtvee |
SpeciesList | scientific name: Rhodotorula toruloides; NCBI TaxID: 5286; |
ModificationList | No PTMs are included in the dataset |
Instrument | LTQ Orbitrap |
Dataset History
Revision | Datetime | Status | ChangeLog Entry |
0 | 2020-05-20 04:15:59 | ID requested | |
⏵ 1 | 2020-07-29 04:20:28 | announced | |
Publication List
Dataset with its publication pending |
Keyword List
submitter keyword: Microbial oil, carotenoids, Rhodotorula toruloides, Genome-scale modelling, xylose, biorefinery, absolute proteomics |
Contact List
Petri-Jaan Lahtvee |
contact affiliation | University of Tartu, Estonia |
contact email | lahtvee@ut.ee |
lab head | |
Petri-Jaan Lahtvee |
contact affiliation | University of Tartu |
contact email | lahtvee@ut.ee |
dataset submitter | |
Full Dataset Link List
Dataset FTP location
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PRIDE project URI |
Repository Record List
[ + ]
[ - ]
- PRIDE
- PXD019305
- Label: PRIDE project
- Name: Xylose Metabolism and the Effect of Oxidative Stress on Lipid and Carotenoid Production in Rhodotorula toruloides: Insights for Future Biorefinery