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PXD019288

PXD019288 is an original dataset announced via ProteomeXchange.

Dataset Summary
TitleCOPPER INDUCES PROTEIN AGGREGATION, A TOXIC PROCESS COMPENSATED BY MOLECULAR CHAPERONES.
DescriptionCopper is a powerful antimicrobial and antiviral agent, which has interest through several biomedical applications. Nevertheless, how copper induces the cellular damage is not fully understood. Two main mechanisms are claimed to be involved, i.e. Cu catalyzed reactive oxygen species production (ROS) and replacements of other essential metal ions, such as Fe in Fe-S clusters by Cu. In the present work, we cumulate strong evidence for the importance of a third mechanism, which proposes that Cu damaging effects are due to its ability to cause massive protein aggregation in a ROS independent mechanism. We further show that Hsp33, a redox-regulated molecular chaperone in Escherichia coli, can prevent Cu-induced protein aggregation, suggesting an important role of the chaperoning system in defense against Cu-toxicity. A closer inspection of the mechanism of how Cu can activate Hsp33 revealed two intriguing features, i) a redox-state specificity of Cu+ vs Cu2+, and ii) an unprecedented mechanism of Hsp33-activation via a non-redox trans-metalation. Indeed, our data suggest that two Cu+ bind to the Zn-Cys4-finger site in Hsp33, which lead to a replacement of one Zn2+ by two Cu+ without Cys oxidation. In contrast, Cu2+ oxidizes the Cys in the Zn-finger, analogue to other oxidative stress effectors of Hsp33. Hence, the present results give several new insights in the antimicrobial mechanism of Cu and how bacteria defend against it.
HostingRepositoryPRIDE
AnnounceDate2022-02-22
AnnouncementXMLSubmission_2022-02-22_04:13:29.309.xml
DigitalObjectIdentifier
ReviewLevelPeer-reviewed dataset
DatasetOriginOriginal dataset
RepositorySupportUnsupported dataset by repository
PrimarySubmitterRosi Fassler
SpeciesList scientific name: Escherichia coli; NCBI TaxID: 562;
ModificationListiodoacetamide derivatized residue
InstrumentQ Exactive HF
Dataset History
RevisionDatetimeStatusChangeLog Entry
02020-05-18 22:22:55ID requested
12022-02-10 08:52:06announced
22022-02-22 04:13:29announced2022-02-22: Updated project metadata.
Publication List
Dataset with its publication pending
Keyword List
submitter keyword: Hsp33, Escherichia coli, molecular chaperone,copper, aggregation
Contact List
Dana Reichmann
contact affiliationDept. of Biological Chemistry, room 1-637 The Alexander Silberman Institute of Life Science The Hebrew University of Jerusalem
contact emaildanare@mail.huji.ac.il
lab head
Rosi Fassler
contact affiliationHebrew University of Jerusalem
contact emailrosi.fassler@mail.huji.ac.il
dataset submitter
Full Dataset Link List
Dataset FTP location
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PRIDE project URI
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