PXD019093

PXD019093 is an original dataset announced via ProteomeXchange.

Title	Human DICER helicase domain recruits PKR and dsRNA binding proteins during viral infection
Description	The innate immune response against viruses mainly involves type I interferon (IFN) in mammalian cells. The exact contribution of the RNA silencing machinery remains to be established, but several recent studies indicate that the type III ribonuclease DICER can generate viral siRNAs in specific conditions. In addition, it has been proposed that type I IFN and RNA silencing could be mutually exclusive responses. In order to decipher the implication of DICER during infection of human cells with the Sindbis virus, we determined its interactome by immunoprecipitation and mass spectrometry analysis. Our results show that human DICER specifically interacts with several double-stranded RNA binding proteins and helicases during viral infection. In particular, proteins such as DHX9, ADAR-1 and the protein kinase PKR are enriched with DICER in virus-infected cells. We validated the importance of the helicase domain of DICER in its interaction with PKR and showed that it has functional consequences for the cellular response to viral infection.
HostingRepository	PRIDE
AnnounceDate	2021-04-15
AnnouncementXML	Submission_2021-04-15_06:25:39.796.xml
DigitalObjectIdentifier	https://dx.doi.org/10.6019/PXD019093
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Supported dataset by repository
PrimarySubmitter	Johana Chicher
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	Phospho; Oxidation; Acetyl; Carbamidomethyl
Instrument	Q Exactive

Revision	Datetime	Status	ChangeLog Entry
0	2020-05-11 08:11:07	ID requested
⏵ 1	2021-04-15 06:25:40	announced

Dataset with its publication pending

submitter keyword: Virus, Alphavirus, RNAi, PKR, DICER, Helicase, Interferon

Sébastien Pfeffer
contact affiliation	Université de Strasbourg, Architecture et Réactivité de l'ARN, Institut de biologie moléculaire et cellulaire du CNRS, 2 allée Konrad Roentgen, 67084 Strasbourg France
contact email	spfeffer@unistra.fr
lab head
Johana Chicher
contact affiliation	Université de Strasbourg, Plateforme Protéomique Strasbourg-Esplanade, Centre National de la Recherche Scientifique
contact email	j.chicher@ibmc-cnrs.unistra.fr
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD019093
     1. Label: PRIDE project
     2. Name: Human DICER helicase domain recruits PKR and dsRNA binding proteins during viral infection