PXD019031 is an
original dataset announced via ProteomeXchange.
Dataset Summary
Title | A Cytoskeletal Protein Complex is Essential for Division of Intracellular Amastigotes of Leishmania mexicana (LAN1416) |
Description | Previous studies in Leishmania mexicana have identified the cytoskeletal protein KHARON as being important for both flagellar trafficking of the glucose transporter GT1 and for successful cytokinesis and survival of infectious amastigote forms inside mammalian macrophages. KHARON is located in three distinct regions of the cytoskeleton: the base of the flagellum, the subpellicular microtubules, and the mitotic spindle. To deconvolve the different functions for KHARON, we have identified two partner proteins, KHAP1 and KHAP2, that associate with KHARON. KHAP1 is located only in the subpellicular microtubules, while KHAP2 is located at the subpellicular microtubules and the base of the flagellum. Both the KHAP1 and KHAP2 null mutants are unable to execute cytokinesis but are able to traffic GT1 to the flagellum. These results confirm that KHARON assembles into distinct functional complexes and that the subpellicular complex is essential for cytokinesis and viability of disease-causing amastigotes but not for flagellar membrane trafficking. |
HostingRepository | PRIDE |
AnnounceDate | 2024-10-22 |
AnnouncementXML | Submission_2024-10-22_05:10:49.102.xml |
DigitalObjectIdentifier | |
ReviewLevel | Peer-reviewed dataset |
DatasetOrigin | Original dataset |
RepositorySupport | Unsupported dataset by repository |
PrimarySubmitter | Phillip Wilmarth |
SpeciesList | scientific name: Leishmania mexicana; NCBI TaxID: 5665; |
ModificationList | monohydroxylated residue |
Instrument | LTQ Velos |
Dataset History
Revision | Datetime | Status | ChangeLog Entry |
0 | 2020-05-07 01:20:12 | ID requested | |
1 | 2020-08-16 22:30:59 | announced | |
⏵ 2 | 2024-10-22 05:10:49 | announced | 2024-10-22: Updated project metadata. |
Publication List
Dataset with its publication pending |
Keyword List
submitter keyword: cytokinesis, cytoskeleton,Leishmania, protein complex, infectious disease, protein targeting |
Contact List
Dr. Scott M. Landfear |
contact affiliation | Department of Molecular Microbiology & Immunology Oregon Health & Science University 3181 SW Sam Jackson Park Road Portland, Oregon 97239, USA |
contact email | landfear@ohsu.edu |
lab head | |
Phillip Wilmarth |
contact affiliation | OHSU |
contact email | wilmarth@ohsu.edu |
dataset submitter | |
Full Dataset Link List
Dataset FTP location
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PRIDE project URI |
Repository Record List
[ + ]
[ - ]
- PRIDE
- PXD019031
- Label: PRIDE project
- Name: A Cytoskeletal Protein Complex is Essential for Division of Intracellular Amastigotes of Leishmania mexicana (LAN1416)