PXD018998
PXD018998 is an original dataset announced via ProteomeXchange.
Dataset Summary
Title | Enabling Routine MHC-II Associated Peptide ProteomicS (MAPPs) for Risk Assessment of Drug-Induced Immunogenicity |
Description | The appended raw files, csv files and other documents were deposited in the public domain in support for the publication Enabling Routine MHC-II Associated Peptide ProteomicS (MAPPs) for Risk Assessment of Drug-Induced Immunogenicity Guido Steiner, Celine Marban-Doran, Jessica Langer, Tatiana Pimenova, Gonzalo Duran-Pacheco; Denise Sauter; Anja Langenkamp, Corinne Solier, Thomas Singer, Katharine Bray-French, and Axel Ducret The abstract is appended below: MHC-II Associated Peptide ProteomicS (MAPPs) is a mass spectrometry-based approach to identify and relatively quantitate naturally processed and presented MHC-II associated peptides that can potentially activate T cells and contribute to the immunogenicity of a drug. Acceptance of the MAPPs technology as an appropriate pre-clinical (and potentially clinical) immunogenicity risk assessment tool depends on its technical stability and robustness but also on the ability to compare results across experiments and donors. To this end, we developed a specialized MAPPs data processing pipeline, dataMAPPs, which presents complex mass spectrometric datasets in the form of heat maps (heatMAPPs) enabling rapid and convenient comparison between conditions and donors. A customized normalization procedure based on identified endogenous peptides standardizes signal intensities within and between donors and enable cross-experimental comparison. We evaluated the technical reproducibility of the MAPPs platform using tool compounds with respect to the most prominent experimental factors and found that the systematic biological differences across donors by far outweighed any technical source of variation. We illustrate the capability of the MAPPs platform to generate data that may be used for pre-clinical risk assessment of drug-induced immunogenicity and discuss its applicability in the clinics. The raw files and associated data for publicly available compounds are appended in the depository. The database (dataMAPPS publication db.FASTA) that was used to search the raw files is also included. The current dataset is divided in three sections, each with explaining documentation: Folder Fig. 2_4_5 contains the raw files, the PEAKS identification files and the quantification files that were used to generate the Fig. 2, 4 and 5 of the publication. The raw files were also used to generate the test files deposited on http://SourceForge.net/projects/datamapps/ which is alsothe source to download the R source code for dataMAPPs. Folder Fig. 6BC contains the raw files, the PEAKS identification files and the quantification files that were used to generate the Fig. 6 BC. The raw files used to generate Fig. 6A were not included; they have been generated using a compound that is proprietary. Folder Fig. 8_KLH contains the raw files, the PEAKS identification files and the quantification files that were used to generate the Fig. 8. |
HostingRepository | MassIVE |
AnnounceDate | 2020-07-21 |
AnnouncementXML | Submission_2020-07-21_03:31:26.xml |
DigitalObjectIdentifier | |
ReviewLevel | Non peer-reviewed dataset |
DatasetOrigin | Original dataset |
RepositorySupport | Unsupported dataset by repository |
PrimarySubmitter | Axel Ducret |
SpeciesList | scientific name: Homo sapiens; common name: human; NCBI TaxID: 9606; scientific name: custom antibody sequences; |
ModificationList | Oxidation; Deamidated; Gln->pyro-Glu |
Instrument | LTQ Orbitrap; Q Exactive |
Dataset History
Revision | Datetime | Status | ChangeLog Entry |
---|---|---|---|
0 | 2020-05-05 03:45:14 | ID requested | |
⏵ 1 | 2020-07-21 03:31:26 | announced |
Publication List
no publication |
Keyword List
submitter keyword: MHC-II peptides, MAPPs, immunogenicity, KLH, infliximab, exotoxin A |
Contact List
Guido Steiner | |
---|---|
contact affiliation | Roche Innovation Center Basel |
contact email | guido.steiner@roche.com |
lab head | |
Axel Ducret | |
contact affiliation | Roche Innovation Center Basel |
contact email | axel.ducret@roche.com |
lab head | |
Axel Ducret | |
contact affiliation | F. Hoffmann-La Roche Ltd |
contact email | axel.ducret@roche.com |
dataset submitter |
Full Dataset Link List
MassIVE dataset URI |
Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://massive.ucsd.edu/MSV000085375/ |