<<< Full experiment listing

PXD018981

PXD018981 is an original dataset announced via ProteomeXchange.

Dataset Summary
TitleStructure of the full kinetoplastids mitoribosome and insight on its large subunit maturation
DescriptionKinetoplastids are unicellular eukaryotic parasites responsible for human pathologies such as Chagas disease, sleeping sickness or Leishmaniasis1. They possess a single large mitochondrion, essential for the parasite survival2. In kinetoplastids mitochondrion, most of the molecular machineries and gene expression processes have significantly diverged and specialized, with the most extreme case being their mitochondrial ribosomes3. These large complexes are in charge of translating the few essential mRNAs encoded by mitochondrial genomes4,5. Structural studies performed in Trypanosoma brucei already highlighted the numerous peculiarities of these mitoribosomes and the maturation of their small subunit3,6. However, several important aspects mainly related to the large subunit remain elusive, such as the structure and maturation of its ribosomal RNA3. Here, we present a cryo-electron microscopy study of the protozoans Leishmania tarentolae and Trypanosoma cruzi mitoribosomes. For both species, we obtained the structure of their mature mitoribosomes, complete rRNA of the large subunit as well as previously unidentified ribosomal proteins. Most importantly, we introduce the structure of an LSU assembly intermediate in presence of 16 identified maturation factors. These maturation factors act both on the intersubunit and solvent sides of the LSU, where they refold and chemically modify the rRNA and prevent early translation before full maturation of the LSU.
HostingRepositoryPRIDE
AnnounceDate2021-04-29
AnnouncementXMLSubmission_2021-04-29_08:11:46.694.xml
DigitalObjectIdentifierhttps://dx.doi.org/10.6019/PXD018981
ReviewLevelPeer-reviewed dataset
DatasetOriginOriginal dataset
RepositorySupportSupported dataset by repository
PrimarySubmitterLauriane Kuhn
SpeciesList scientific name: Trypanosoma cruzi cruzi; NCBI TaxID: 85057; scientific name: Leishmania tarentolae; NCBI TaxID: 5689;
ModificationListPhospho; Oxidation; Acetyl; Sulfo; Carbamidomethyl
InstrumentQ Exactive
Dataset History
RevisionDatetimeStatusChangeLog Entry
02020-05-04 17:51:23ID requested
12021-04-29 08:11:47announced
Publication List
Dataset with its publication pending
Keyword List
submitter keyword: mitochondria, ribosome, mitoribosome, kinetoplastids, trypanosoma, leishmania
Contact List
Florent Waltz
contact affiliationInstitut Européen de Chimie et Biologie (IECB), U1212 Inserm, Université de Bordeaux, 2 rue R. Escarpit, F-33600 Pessac, France
contact emailwaltz.florent@gmail.com
lab head
Lauriane Kuhn
contact affiliationCNRS, IBMC, FRC1589
contact emaill.kuhn@ibmc-cnrs.unistra.fr
dataset submitter
Full Dataset Link List
Dataset FTP location
NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2021/04/PXD018981
PRIDE project URI
Repository Record List
[ + ]