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PXD018959

PXD018959 is an original dataset announced via ProteomeXchange.

Dataset Summary
TitleA mass spectrometry-based antibody free approach enables the quantification of D-dimer in plasma
DescriptionD-dimer is an important marker of different coagulation disease, such as venous thromboembolism (including deep vein thrombosis and pulmonary embolism) and disseminated intravascular coagulation. Though it is frequently used in many clinical diagnostic situations, the D-dimer assays currently lack standardization due to its inherent heterogeneity which makes the antibody-based methods have different quantitative results and cutoffs to define an abnormal value. In this study, we report the first antibody-free D-dimer quantification method. In the method, a cross-linked peptide of fibrin D domain carboxyl terminal cross-linked by the factor XIIIa was used to represent the D-dimer. By using a filter-aided sample preparation and nickel immobilized metal affinity chromatography enrichment strategy, the complexity of the plasma sample was significantly reduced and the cross-linked peptide was enriched effectively for analysis with parallel reaction monitoring in mass spectrometry. The linear range of this method was 3.125nmol/L to 400nmol/L which spanning over two magnitudes. Recovery and reproducibility of the method were found to be good. To further demonstrate the performance of our method, D-dimer concentrations of twenty-five human plasma were analyzed and the results had a good correlation between with the commercial D-dimer assay kit used in hospital. This method was completely antibody-free and have potential to promote the standardization of D-dimer analysis.
HostingRepositoryjPOST
AnnounceDate2021-05-04
AnnouncementXMLSubmission_2022-09-18_04:02:50.587.xml
DigitalObjectIdentifier
ReviewLevelNon peer-reviewed dataset
DatasetOriginOriginal dataset
RepositorySupportUnsupported dataset by repository
PrimarySubmitterNa Zhang
SpeciesList scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationListL-methionine sulfoxide; S-carboxamidomethyl-L-cysteine
InstrumentQ Exactive
Dataset History
RevisionDatetimeStatusChangeLog Entry
02020-05-03 22:22:55ID requested
12021-05-03 08:00:06announced
22022-09-18 04:02:50announced2022-09-18: Updated FTP location.
Publication List
Dataset with its publication pending
Keyword List
submitter keyword: D-dimer, antibody free, nickel immobilized metal affinity chromatography, parallel reaction monitoring-mass spectrometry, filter-aided sample preparation, quantification
Contact List
Mingliang Ye
lab head
Na Zhang
contact affiliationDalian Institute of Chemical Physics, Chinese Academy of Sciences
dataset submitter
Full Dataset Link List
jPOST dataset URI
Dataset FTP location
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