PXD018915

PXD018915 is an original dataset announced via ProteomeXchange.

Title	Heat-induced proteomes in tomato pollen mother cells
Description	Tomato pollen production and viability is highly vulnerable to higher temperature. Hot summers with temperature reaching above 32°C can disrupt production of viable pollens and fruit set, resulting in yield loss. In recent years, temperature above 35-38oC has become a norm during mid-summer with potential adverse impacts on the production of tomatoes and many other crop species. Pollens are developed through the microsporogenesis and micro-gametogenesis stages. The most heat sensitive period is from the meiotic process of the microsporocytes, at the young microspore stage (uninucleate stage of microspore) to during late pollen development (pollen mitosis). This project studied the heat-induced proteomes in microsporocyte, also called pollen mother cells (PMC). Homogenous PMC samples were collected from cross-sectioned frozen fresh anther tissues of tomato ‘Maxifort’ using laser capture microdissection (LCM). Tandem mass tag (TMT) proteomics analysis was conducted to identify proteomics changes related to heat tolerance during pollen development.
HostingRepository	PRIDE
AnnounceDate	2022-04-07
AnnouncementXML	Submission_2022-04-07_05:48:23.181.xml
DigitalObjectIdentifier	https://dx.doi.org/10.6019/PXD018915
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Supported dataset by repository
PrimarySubmitter	Ted Thannhauser
SpeciesList	scientific name: Solanum lycopersicum; NCBI TaxID: 4081;
ModificationList	Methylthio; Deamidated; TMT6plex; Oxidation
Instrument	LTQ Orbitrap

Revision	Datetime	Status	ChangeLog Entry
0	2020-04-29 16:37:16	ID requested
⏵ 1	2022-04-07 05:48:23	announced

Dataset with its publication pending

submitter keyword: pollen mother cells, Laser Capture Microdissection, TMT proteomics, heat stress Project description

Theodore Thannhauser
contact affiliation	USDA-ARS
contact email	tt34@cornell.edu
lab head
Ted Thannhauser
contact affiliation	USDA-ARS
contact email	tt34@cornell.edu
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD018915
     1. Label: PRIDE project
     2. Name: Heat-induced proteomes in tomato pollen mother cells