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PXD018662

PXD018662 is an original dataset announced via ProteomeXchange.

Dataset Summary
TitleUSP9X controls the resolution of stalled ribosomes
DescriptionWhen a ribosome stalls during translation, it runs the risk of collision with a trailing ribosome. Such an encounter leads to the formation of a stable di-ribosome complex, which needs to be resolved by a dedicated machinery. The initial stalling and the subsequent resolution of di-ribosomal complexes requires activity of Makorin and ZNF598 ubiquitin E3 ligases respectively, through ubiquitylation of the eS10 and uS10 sub-units of the ribosome. It is common for the stability of RING E3 ligases to be regulated by an interacting deubiquitylase, which often opposes auto-ubiquitylation of the E3. Here, we show that the deubiquitylase USP9X directly interacts with ZNF598 and regulates its abundance through the control of protein stability. We have developed a highly specific small molecule inhibitor of USP9X. In the absence of USP9X or following chemical inhibition of its catalytic activity, steady state levels of Makorins and ZNF598 are diminished and the ribosomal quality control pathway is impaired.
HostingRepositoryPRIDE
AnnounceDate2024-10-22
AnnouncementXMLSubmission_2024-10-22_05:18:05.851.xml
DigitalObjectIdentifier
ReviewLevelPeer-reviewed dataset
DatasetOriginOriginal dataset
RepositorySupportUnsupported dataset by repository
PrimarySubmitterAdan Pinto-Fernandez
SpeciesList scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationListNo PTMs are included in the dataset
InstrumentQ Exactive HF
Dataset History
RevisionDatetimeStatusChangeLog Entry
02020-04-19 09:55:36ID requested
12021-01-25 22:38:09announced
22024-10-22 05:18:06announced2024-10-22: Updated project metadata.
Publication List
Dataset with its publication pending
Keyword List
submitter keyword: ZNF598, ribosomal stalling, Makorin,ubiquitin, deubiquitylase, USP9X, DUB inhibitor, translation
Contact List
Prof. Benedikt Kessler
contact affiliationTarget Discovery Institute - Nuffield Department of medicine (University of Oxford)
contact emailbenedikt.kessler@ndm.ox.ac.uk
lab head
Adan Pinto-Fernandez
contact affiliationUniversity of Oxford
contact emailadan.pintofernandez@ndm.ox.ac.uk
dataset submitter
Full Dataset Link List
Dataset FTP location
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PRIDE project URI
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