PXD018382 is an
original dataset announced via ProteomeXchange.
Dataset Summary
Title | Mycobacteria tolerate carbon monoxide by remodelling their respiratory chain |
Description | Carbon monoxide (CO) is a gas infamous for its acute toxicity. The toxicity of CO predominately stems from its tendency to form carbonyl complexes with transition metals, thus inhibiting the heme-prosthetic groups of proteins, including the terminal oxidases of the respiratory chain. While CO has been proposed as an antibacterial agent, the evidence supporting its toxicity towards bacteria is equivocal, and its cellular targets remain poorly defined. In this work, we investigate the physiological response of Mycobacterium smegmatis to CO. We show that M. smegmatis is highly resistant to the toxic effects of CO, exhibiting normal growth parameters when cultured in its presence. We profiled the proteome of M. smegmatis during growth in CO, identifying strong induction of cytochrome bd oxidase and members of the dos regulon, but relatively few other changes. We show that the activity of cytochrome bd oxidase is resistant to CO, whereas cytochrome bcc-aa3 oxidase is strongly inhibited by this gas. Consistent with these findings, growth analysis shows that M. smegmatis lacking cytochrome bd oxidase displays a significant growth defect in the presence of CO, while induction of the dos regulon appears to be unimportant for adaption to CO. Altogether, our findings suggest that M. smegmatis has considerable resistance to CO and benefits from respiratory flexibility to withstand its inhibitory effects. |
HostingRepository | PRIDE |
AnnounceDate | 2021-04-30 |
AnnouncementXML | Submission_2021-04-30_00:33:10.124.xml |
DigitalObjectIdentifier | |
ReviewLevel | Peer-reviewed dataset |
DatasetOrigin | Original dataset |
RepositorySupport | Unsupported dataset by repository |
PrimarySubmitter | Cheng Huang |
SpeciesList | scientific name: Mycobacterium sp. GN-10803; NCBI TaxID: 632138; |
ModificationList | No PTMs are included in the dataset |
Instrument | Q Exactive HF; Orbitrap Fusion |
Dataset History
Revision | Datetime | Status | ChangeLog Entry |
0 | 2020-04-06 01:21:06 | ID requested | |
⏵ 1 | 2021-04-30 00:33:10 | announced | |
Publication List
Dataset with its publication pending |
Keyword List
submitter keyword: Carbon monoxide, respiratory chain, Mycobacteria |
Contact List
Chris Greening |
contact affiliation | Department of Microbiology, Monash Biomedicine Discovery Institute, Monash University, Clayton, VIC 3800, Australia |
contact email | chris.greening@monash.edu |
lab head | |
Cheng Huang |
contact affiliation | Monash University |
contact email | cheng.huang@monash.edu |
dataset submitter | |
Full Dataset Link List
Dataset FTP location
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PRIDE project URI |
Repository Record List
[ + ]
[ - ]
- PRIDE
- PXD018382
- Label: PRIDE project
- Name: Mycobacteria tolerate carbon monoxide by remodelling their respiratory chain