PXD018295 is an
original dataset announced via ProteomeXchange.
Dataset Summary
Title | Improvements on the quantitative analysis of Trypanosoma cruzi histone post translational modifications |
Description | Trypanosome histone N-terminal sequences are very divergent from the other eukaryotes, although they are still decorated by post-translational modifications (PTMs). Here, we used a highly robust workflow to analyze histone PTMs in the parasite Trypanosoma cruzi using mass spectrometry-based data-independent acquisition (DIA). We adapted the workflow for the analysis of the parasite’s histone sequences by modifying the software EpiProfile 2.0, improving peptide and PTM quantification accuracy. This workflow could now be applied to the study of 141 T. cruzi modified histone peptides, which we used to investigate the dynamics of histone PTMs along the metacyclogenesis and the life cycle of T. cruzi. |
HostingRepository | PRIDE |
AnnounceDate | 2024-10-07 |
AnnouncementXML | Submission_2024-10-07_13:33:10.019.xml |
DigitalObjectIdentifier | |
ReviewLevel | Peer-reviewed dataset |
DatasetOrigin | Original dataset |
RepositorySupport | Unsupported dataset by repository |
PrimarySubmitter | Julia Cunha |
SpeciesList | scientific name: Trypanosoma cruzi cruzi; NCBI TaxID: 85057; |
ModificationList | monomethylated residue; phosphorylated residue; acetylated residue; dimethylated residue; N6,N6,N6-trimethyl-L-lysine |
Instrument | LTQ Orbitrap Velos |
Dataset History
Revision | Datetime | Status | ChangeLog Entry |
0 | 2020-03-30 23:40:07 | ID requested | |
⏵ 1 | 2024-10-07 13:33:10 | announced | |
Publication List
Dataset with its publication pending |
Keyword List
submitter keyword: PTM, Trypanosoma cruzi,Histone |
Contact List
Julia da Cunha |
contact affiliation | Cell Cycle Laboratory, Butantan Institute, Brazil |
contact email | julia.cunha@butantan.gov.br |
lab head | |
Julia Cunha |
contact affiliation | LCC |
contact email | julia.cunha@butantan.gov.br |
dataset submitter | |
Full Dataset Link List
Dataset FTP location
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PRIDE project URI |
Repository Record List
[ + ]
[ - ]
- PRIDE
- PXD018295
- Label: PRIDE project
- Name: Improvements on the quantitative analysis of Trypanosoma cruzi histone post translational modifications