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PXD018295

PXD018295 is an original dataset announced via ProteomeXchange.

Dataset Summary
TitleImprovements on the quantitative analysis of Trypanosoma cruzi histone post translational modifications
DescriptionTrypanosome histone N-terminal sequences are very divergent from the other eukaryotes, although they are still decorated by post-translational modifications (PTMs). Here, we used a highly robust workflow to analyze histone PTMs in the parasite Trypanosoma cruzi using mass spectrometry-based data-independent acquisition (DIA). We adapted the workflow for the analysis of the parasite’s histone sequences by modifying the software EpiProfile 2.0, improving peptide and PTM quantification accuracy. This workflow could now be applied to the study of 141 T. cruzi modified histone peptides, which we used to investigate the dynamics of histone PTMs along the metacyclogenesis and the life cycle of T. cruzi.
HostingRepositoryPRIDE
AnnounceDate2024-10-07
AnnouncementXMLSubmission_2024-10-07_13:33:10.019.xml
DigitalObjectIdentifier
ReviewLevelPeer-reviewed dataset
DatasetOriginOriginal dataset
RepositorySupportUnsupported dataset by repository
PrimarySubmitterJulia Cunha
SpeciesList scientific name: Trypanosoma cruzi cruzi; NCBI TaxID: 85057;
ModificationListmonomethylated residue; phosphorylated residue; acetylated residue; dimethylated residue; N6,N6,N6-trimethyl-L-lysine
InstrumentLTQ Orbitrap Velos
Dataset History
RevisionDatetimeStatusChangeLog Entry
02020-03-30 23:40:07ID requested
12024-10-07 13:33:10announced
Publication List
Dataset with its publication pending
Keyword List
submitter keyword: PTM, Trypanosoma cruzi,Histone
Contact List
Julia da Cunha
contact affiliationCell Cycle Laboratory, Butantan Institute, Brazil
contact emailjulia.cunha@butantan.gov.br
lab head
Julia Cunha
contact affiliationLCC
contact emailjulia.cunha@butantan.gov.br
dataset submitter
Full Dataset Link List
Dataset FTP location
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PRIDE project URI
Repository Record List
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