PXD018153 is an
original dataset announced via ProteomeXchange.
Dataset Summary
Title | Proteome dynamics in persisting and resuscitating bacterial cells |
Description | Bacterial persister cells become transiently tolerant to antibiotics by restraining their growth and metabolic activity. Detailed molecular characterization of bacterial persistence is hindered by low count of persisting cells and the need for their isolation. Here we used sustained addition of stable isotope-labeled lysine to selectively label the proteome of hipA-induced persisting and hipB-induced resuscitating E. coli cells in minimal medium after antibiotic treatment. Time-resolved, 24-hour measurement of label incorporation allowed detection of more than 500 newly synthetized proteins in persister cells, demonstrating low but widespread protein synthesis during persistence. Many essential proteins were newly synthesized; several ribosome-associated proteins showed unusually high synthesis levels and are involved in maintenance of persistence. At the onset of resuscitation, cells synthesized ABC transporters, restored translation machinery and resumed metabolism by inducing glycolysis and biosynthesis of amino acids. This dataset provides an unprecedented insight into the processes governing persistence and resuscitation of bacterial cells. |
HostingRepository | PRIDE |
AnnounceDate | 2024-10-22 |
AnnouncementXML | Submission_2024-10-22_05:24:20.615.xml |
DigitalObjectIdentifier | |
ReviewLevel | Peer-reviewed dataset |
DatasetOrigin | Original dataset |
RepositorySupport | Unsupported dataset by repository |
PrimarySubmitter | Nicolas Nalpas |
SpeciesList | scientific name: Escherichia coli; NCBI TaxID: 562; |
ModificationList | No PTMs are included in the dataset |
Instrument | Q Exactive |
Dataset History
Revision | Datetime | Status | ChangeLog Entry |
0 | 2020-03-23 09:29:52 | ID requested | |
1 | 2021-07-30 00:33:12 | announced | |
⏵ 2 | 2024-10-22 05:24:21 | announced | 2024-10-22: Updated project metadata. |
Publication List
Dataset with its publication pending |
Keyword List
submitter keyword: hipB, resuscitation, hipA, persistence,Escherichia coli |
Contact List
Boris Macek |
contact affiliation | Quantitative Proteomics & Proteome Center Tuebingen Interfaculty Institute for Cell Biology Department of Biology University of Tuebingen Auf der Morgenstelle 15 72076 Tuebingen Germany |
contact email | boris.macek@uni-tuebingen.de |
lab head | |
Nicolas Nalpas |
contact affiliation | University of Rouen |
contact email | nalpas.nicolas@gmail.com |
dataset submitter | |
Full Dataset Link List
Dataset FTP location
NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2021/07/PXD018153 |
PRIDE project URI |
Repository Record List
[ + ]
[ - ]
- PRIDE
- PXD018153
- Label: PRIDE project
- Name: Proteome dynamics in persisting and resuscitating bacterial cells