PXD018034

PXD018034 is an original dataset announced via ProteomeXchange.

Title	Maturation kinetics of a multiprotein complex revealed by metabolic labeling
Description	The project aims at computing in vivo co-assembly kinetics from metabolomic-inspired approaches. In short, we performed reciprocal pull-down experiments from complex cellular components at different time points following pulsed-SILAC metabolic labeling. We applied the method to 320 pairs of yeast nucleoporin (NUP) proteins constituting the ~ 50 MDa Nuclear Pore Complex (NPC). Our analysis reveals a hierarchical principle of the NPC biogenesis where individual subcomplexes form on a minute time-scale, followed by their co-assembly from central to peripheral subunits in a ~ 1 hour long maturation process.
HostingRepository	PRIDE
AnnounceDate	2020-10-16
AnnouncementXML	Submission_2020-10-16_01:19:48.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Ludovic Gillet
SpeciesList	scientific name: Saccharomyces cerevisiae (Baker's yeast); NCBI TaxID: 4932;
ModificationList	iodoacetamide derivatized residue
Instrument	Orbitrap Fusion Lumos; Q Exactive

Revision	Datetime	Status	ChangeLog Entry
0	2020-03-13 01:28:54	ID requested
⏵ 1	2020-10-16 01:19:48	announced

Dataset with its publication pending

submitter keyword: yeast, nuclear pore complex, maturation kinetics, pulsed SILAC, data independent acquisition

Evgeny Onischenko
contact affiliation	Department of Biological Sciences, University of Bergen, Bergen, Norway
contact email	evgeny.onishchenko@uib.no
lab head
Ludovic Gillet
contact affiliation	ETH Zurich
contact email	gillet@imsb.biol.ethz.ch
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2020/10/PXD018034

PRIDE project URI

• PRIDE
  1. PXD018034
     1. Label: PRIDE project
     2. Name: Maturation kinetics of a multiprotein complex revealed by metabolic labeling