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PXD017721

PXD017721 is an original dataset announced via ProteomeXchange.

Dataset Summary
TitleProteomics profiling of KAIMRC1 in comparison to MDA-MB231 and MCF-7
DescriptionProteomics characterization of KAIMRC1 cell line, a naturally immortalized breast cancer cells, is described in comparison to MCF7 and MDA-231 breast cancer cells. Quantitative proteomics analysis using the tandem mass tag (TMT) labeled technique in conjunction with phosphopeptide enrichment method was used to perform comparative profiling of proteins and phosphoproteins in the three cell lines. In total, 673 proteins and 33 Phosphoproteins were differentially expressed among these cell lines. These proteins are involved in several key cellular pathways that include DNA replication and repair, Splicing machinery, amino acid metabolism, cellular energy, and Estrogen signaling pathway. Many of the differentially expressed proteins are associated with different types of tumors including breast cancer. For validation, 4 highly significant expressed proteins including MTAP, KCTD12, PARP 1, and Prelamin-A/C were subjected to western blotting and the results were consistent with proteomics analysis. Unlike MCF-7 and MDA-MB-231, KAIMRC1 showed different phospho- and non-phosphoproteomic phenotypes which make it a potential model to study breast cancer.
HostingRepositoryPRIDE
AnnounceDate2020-06-22
AnnouncementXMLSubmission_2020-06-21_22:24:19.xml
DigitalObjectIdentifier
ReviewLevelPeer-reviewed dataset
DatasetOriginOriginal dataset
RepositorySupportUnsupported dataset by repository
PrimarySubmitterBandar Alghanem
SpeciesList scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationListTMT6plex-126 reporter+balance reagent acylated residue
InstrumentOrbitrap Fusion
Dataset History
RevisionDatetimeStatusChangeLog Entry
02020-02-25 07:21:15ID requested
12020-06-21 22:24:19announced
Publication List
Dataset with its publication pending
Keyword List
submitter keyword: Breast cancer
cell lines
TMT
Proteomics
Phosphoproteomics
Contact List
Mohamed Boudjelal
contact affiliationKing Abdullah international medical research center
contact emailboudjelalmo@ngha.med.sa
lab head
Bandar Alghanem
contact affiliationking abdullah international medical research center
contact emailghanembandar@gmail.com
dataset submitter
Full Dataset Link List
Dataset FTP location
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