PXD017721 is an
original dataset announced via ProteomeXchange.
Dataset Summary
Title | Proteomics profiling of KAIMRC1 in comparison to MDA-MB231 and MCF-7 |
Description | Proteomics characterization of KAIMRC1 cell line, a naturally immortalized breast cancer cells, is described in comparison to MCF7 and MDA-231 breast cancer cells. Quantitative proteomics analysis using the tandem mass tag (TMT) labeled technique in conjunction with phosphopeptide enrichment method was used to perform comparative profiling of proteins and phosphoproteins in the three cell lines. In total, 673 proteins and 33 Phosphoproteins were differentially expressed among these cell lines. These proteins are involved in several key cellular pathways that include DNA replication and repair, Splicing machinery, amino acid metabolism, cellular energy, and Estrogen signaling pathway. Many of the differentially expressed proteins are associated with different types of tumors including breast cancer. For validation, 4 highly significant expressed proteins including MTAP, KCTD12, PARP 1, and Prelamin-A/C were subjected to western blotting and the results were consistent with proteomics analysis. Unlike MCF-7 and MDA-MB-231, KAIMRC1 showed different phospho- and non-phosphoproteomic phenotypes which make it a potential model to study breast cancer. |
HostingRepository | PRIDE |
AnnounceDate | 2020-06-22 |
AnnouncementXML | Submission_2020-06-21_22:24:19.xml |
DigitalObjectIdentifier | |
ReviewLevel | Peer-reviewed dataset |
DatasetOrigin | Original dataset |
RepositorySupport | Unsupported dataset by repository |
PrimarySubmitter | Bandar Alghanem |
SpeciesList | scientific name: Homo sapiens (Human); NCBI TaxID: 9606; |
ModificationList | TMT6plex-126 reporter+balance reagent acylated residue |
Instrument | Orbitrap Fusion |
Dataset History
Revision | Datetime | Status | ChangeLog Entry |
0 | 2020-02-25 07:21:15 | ID requested | |
⏵ 1 | 2020-06-21 22:24:19 | announced | |
Publication List
Dataset with its publication pending |
Keyword List
submitter keyword: Breast cancer |
cell lines |
TMT |
Proteomics |
Phosphoproteomics |
Contact List
Mohamed Boudjelal |
contact affiliation | King Abdullah international medical research center |
contact email | boudjelalmo@ngha.med.sa |
lab head | |
Bandar Alghanem |
contact affiliation | king abdullah international medical research center |
contact email | ghanembandar@gmail.com |
dataset submitter | |
Full Dataset Link List
Dataset FTP location
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PRIDE project URI |
Repository Record List
[ + ]
[ - ]
- PRIDE
- PXD017721
- Label: PRIDE project
- Name: Proteomics profiling of KAIMRC1 in comparison to MDA-MB231 and MCF-7