PXD017696 is an
original dataset announced via ProteomeXchange.
Dataset Summary
Title | Comprehensive quantitative analysis of the proteome of Leishmania (Viannia) species |
Description | Leishmania species are responsible for a broad spectrum of diseases, denominated Leishmaniasis, affecting over 12 million people worldwide. During the last decade, there have been impressive efforts for sequencing the genome of most of the pathogenic Leishmania spp. as well as hundreds of strains, but large-scale proteomic analyses did not follow these achievements and Leishmania proteome remained mostly uncharacterized. Here, we report a comprehensive comparative study of the proteomes of L. braziliensis, L. panamensis and L. guyanensis. Proteins extracted by SDS-mediated lysis were processed following the multi-enzyme digestion-filter aided sample preparation (FASP) procedure and analysed by high accuracy mass spectrometry. “Total Protein Approach” and “Proteomic Ruler” were applied for absolute quantification of proteins. Principal component analysis demonstrated very high reproducibility among biological replicates and a very clear differentiation of the three species. Our dataset comprises near 7000 proteins, representing the most complete Leishmania proteome yet known, and provides a quantitative picture of the proteomes of the three species in terms of protein concentration and copy numbers. We describe the abundance of proteins from the major energy metabolic processes, highlighting the main differences among the species. In addition, we provide quantitative data about different membrane proteins, transporters, and lipids, all of which delineate significant differences among those species and provide rich substrate for explore new molecules for diagnosing purposes. |
HostingRepository | PRIDE |
AnnounceDate | 2020-08-10 |
AnnouncementXML | Submission_2020-08-09_23:12:12.xml |
DigitalObjectIdentifier | |
ReviewLevel | Peer-reviewed dataset |
DatasetOrigin | Original dataset |
RepositorySupport | Unsupported dataset by repository |
PrimarySubmitter | Jacek Wisniewski |
SpeciesList | scientific name: Leishmania guyanensis species complex; NCBI TaxID: 38579; |
ModificationList | No PTMs are included in the dataset |
Instrument | Q Exactive |
Dataset History
Revision | Datetime | Status | ChangeLog Entry |
0 | 2020-02-24 00:33:06 | ID requested | |
⏵ 1 | 2020-08-09 23:12:12 | announced | |
Publication List
Dataset with its publication pending |
Keyword List
submitter keyword: Leishmania (Viannia) |
Leishmania braziliensis |
Leishmania panamensis |
Leishmania guyanensis |
proteomics |
label-free quantification |
Filter Aided Sample Preparation, Total Protein Approach, Proteomic Ruler, mass spectrometry |
Contact List
Jacek R Wisniewski |
contact affiliation | Max Planck Institute of Biochemistry Martinsried, Germany |
contact email | jwisniew@biochem.mpg.de |
lab head | |
Jacek Wisniewski |
contact affiliation | Max Planck Institute of Biochemistry Martinsried, Germany |
contact email | jwisniew@biochem.mpg.de |
dataset submitter | |
Full Dataset Link List
Dataset FTP location
NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2020/08/PXD017696 |
PRIDE project URI |
Repository Record List
[ + ]
[ - ]
- PRIDE
- PXD017696
- Label: PRIDE project
- Name: Comprehensive quantitative analysis of the proteome of Leishmania (Viannia) species