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PXD017622

PXD017622 is an original dataset announced via ProteomeXchange.

Dataset Summary
TitleA divergent cyclin/cyclin-dependent kinase complex controls the atypical replication of Plasmodium berghei during gametogony and parasite transmission.
DescriptionCell cycle transitions are generally triggered by variation in the activity of cyclin-dependent kinases (CDKs) bound to cyclins. Malaria-causing parasites have a life cycle with unique cell-division cycles, and a repertoire of divergent CDKs and cyclins of poorly understood function and interdependency. We show that Plasmodium berghei CDK-related kinase 5 (CRK5), is a critical regulator of atypical mitosis in the gametogony and is required for mosquito transmission. It phosphorylates canonical CDK motifs of components in the pre-replicative complex and is essential for DNA replication. We also provide evidence for indirect regulation of the concomitant M-phase progression. During a replicative cycle, CRK5 stably interacts with a single Plasmodium-specific cyclin (SOC2), although we obtained no evidence of SOC2 cycling by transcription, translation or degradation. Our results provide evidence that during Plasmodium male gametogony, this unique cyclin/CDK pair fills the functional space of multiple eukaryotic cell-cycle kinases controlling DNA replication and M-phase progression.
HostingRepositoryPRIDE
AnnounceDate2020-06-17
AnnouncementXMLSubmission_2020-06-16_23:01:48.xml
DigitalObjectIdentifierhttps://dx.doi.org/10.6019/PXD017622
ReviewLevelPeer-reviewed dataset
DatasetOriginOriginal dataset
RepositorySupportSupported dataset by repository
PrimarySubmitterAndrew Bottrill
SpeciesList scientific name: Plasmodium berghei ANKA; NCBI TaxID: 5823;
ModificationListAmmonia-loss; Dehydrated; Oxidation; Acetyl; acetylated residue; monohydroxylated residue; Carbamidomethyl; iodoacetamide derivatized residue
InstrumentOrbitrap Fusion
Dataset History
RevisionDatetimeStatusChangeLog Entry
02020-02-19 07:43:16ID requested
12020-06-16 23:01:48announced
Publication List
Dataset with its publication pending
Keyword List
submitter keyword: Malaria, Plasmodium, Cyclin, Cyclin-Dependent, kinase, parasite, Warwick-RTP
Contact List
Dr. Rita Tewari
contact affiliationSchool of Life Sciences, Queens Medical Centre, University of Nottingham, Nottingham, NG7 2UH
contact emailrita.tewari@nottingham.ac.uk
lab head
Andrew Bottrill
contact affiliationUniversity of Warwick
contact emaila.r.bottrill@gmail.com
dataset submitter
Full Dataset Link List
Dataset FTP location
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PRIDE project URI
Repository Record List
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