PXD017388
PXD017388 is an original dataset announced via ProteomeXchange.
Dataset Summary
Title | Reproducible label-free quantitative proteomics profiling cellular responses to engineered nanomaterials |
Description | Proteomic data from engineered nanomaterial- treated THP-1 cells. Time points at 12 hours; 4 biological replicates for each treatment. Human THP-1 cells were either mock-treated (control) or treated with engineered nanomaterials (ENMs). Four biological replicates were used for each sample group (Control, or ENM treatment at either the low or high dosage). In the block design scheme for LC-MS analysis, samples treated with two different ENMs were assign into an analytical block (2 ENM X 2 dosages/ENM X 4 replicates = 16 samples). Four control samples were also included in each block, yielding 20 samples per block. Blocks of samples were divided into two processing batches. Samples in each batch were processed simultaneously from cell culture, to treatment with ENMs, and finally to proteomic sample preparation for LC-MS analysis (including trypsin digestion). |
HostingRepository | MassIVE |
AnnounceDate | 2020-02-27 |
AnnouncementXML | Submission_2020-02-27_14:06:39.xml |
DigitalObjectIdentifier | |
ReviewLevel | Peer-reviewed dataset |
DatasetOrigin | Original dataset |
RepositorySupport | Supported dataset by repository |
PrimarySubmitter | Matthew Monroe |
SpeciesList | scientific name: Homo sapiens; common name: human; NCBI TaxID: 9606; |
ModificationList | Carbamidomethyl; Oxidation |
Instrument | Q Exactive |
Dataset History
Revision | Datetime | Status | ChangeLog Entry |
---|---|---|---|
0 | 2020-02-05 00:17:42 | ID requested | |
⏵ 1 | 2020-02-27 14:06:40 | announced |
Publication List
Tong Zhang, Matthew J. Gaffrey, Dennis G. Thomas, Thomas J. Weber, Becky M. Hess, Karl K.Weitz, Paul D. Piehowski, Vladislav A. Petyuk, Ronald J. Moore, Wei-Jun Qian, and Brian D. Thrall. A proteome-wide assessment of the oxidative stress paradigm for metal and metal-oxide nanomaterials in human macrophages. NanoImpact. 2020 Jan |
17: 100194. doi: 10.1016/j.impact.2019.100194. |
Keyword List
submitter keyword: proteomics, label-free quantification, large cohort |
Contact List
Wei-Jun Qian | |
---|---|
contact affiliation | Pacific Northwest National Laboratory |
contact email | Weijun.Qian@pnnl.gov |
lab head | |
Matthew Monroe | |
contact affiliation | Pacific Northwest National Laboratory |
contact email | matthew.monroe@pnnl.gov |
dataset submitter |
Full Dataset Link List
MassIVE dataset URI |
Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://massive.ucsd.edu/MSV000084903/ |