PXD017277 is an
original dataset announced via ProteomeXchange.
Dataset Summary
Title | Succession of Bifidobacterium longum strains in response to a changing early life nutritional environment reveals dietary substrate adaptations |
Description | Diet-microbe interactions play a crucial role in infant development and modulation of the early-life microbiota. The genus Bifidobacterium dominates the breast-fed infant gut, with strains of B. longum subsp. longum (B. longum) and B. longum subsp. infantis (B. infantis) particularly prevalent within the early-life microbiota. Although, transition from milk to a more diversified diet later in infancy initiates a shift to a more complex microbiome, with concurrent reductions in Bifidobacterium abundance, specific strains of B. longum may persist in individual hosts for prolonged periods of time. Here, we sought to investigate the adaptation of B. longum to the changing infant diet during the early-life developmental window. Genomic characterisation of 75 strains isolated from nine either exclusively breast- or formula-fed infants in the first 18 months of their lives revealed subspecies- and strain-specific intra-individual genomic diversity with respect to glycosyl hydrolase families and enzymes, which corresponded to different dietary stages. Complementary phenotypic growth studies indicated strain-specific differences in human milk oligosaccharide and plant carbohydrate utilisation profiles between and within individual infants, while proteomic profiling identified proteins involved in metabolism of selected carbohydrates. Our results indicate a strong link between infant diet and B. longum subspecies/strain genomic and carbohydrate utilisation diversity, which aligns with a changing nutritional environment i.e. moving from breast milk to a solid food diet. These data provide additional insights into possible mechanisms responsible for the competitive advantage of this bifidobacterial species and their long-term persistence in a single host and may contribute to rational development of new dietary therapies for this important development window. |
HostingRepository | PRIDE |
AnnounceDate | 2024-10-22 |
AnnouncementXML | Submission_2024-10-22_05:09:02.524.xml |
DigitalObjectIdentifier | |
ReviewLevel | Peer-reviewed dataset |
DatasetOrigin | Original dataset |
RepositorySupport | Unsupported dataset by repository |
PrimarySubmitter | Sabina Leanti La Rosa |
SpeciesList | scientific name: Bifidobacterium longum subsp. longum; NCBI TaxID: 1679; |
ModificationList | monohydroxylated residue; acetylated residue; iodoacetamide derivatized residue; deamidated residue |
Instrument | Q Exactive HF |
Dataset History
Revision | Datetime | Status | ChangeLog Entry |
0 | 2020-01-24 06:27:24 | ID requested | |
1 | 2020-07-15 01:04:13 | announced | |
⏵ 2 | 2024-10-22 05:09:02 | announced | 2024-10-22: Updated project metadata. |
Publication List
Dataset with its publication pending |
Keyword List
submitter keyword: cellobiose,Bifidobacterium, 2'fucosyllactose, lactoneotetraose |
Contact List
Phil B. Pope |
contact affiliation | Norwegian University of Life Sciences, Aas, Norway |
contact email | phil.pope@nmbu.no |
lab head | |
Sabina Leanti La Rosa |
contact affiliation | Faculty of Chemistry, Biotechnology and Food Science, Norwegian University of Life Sciences |
contact email | sabina.leantilarosa@nmbu.no |
dataset submitter | |
Full Dataset Link List
Dataset FTP location
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PRIDE project URI |
Repository Record List
[ + ]
[ - ]
- PRIDE
- PXD017277
- Label: PRIDE project
- Name: Succession of Bifidobacterium longum strains in response to a changing early life nutritional environment reveals dietary substrate adaptations