PXD017171

PXD017171 is an original dataset announced via ProteomeXchange.

Dataset Summary

Title	Comprehensive functional analysis of Escherichia coli ribosomal RNA methyltransferases
Description	We analyzed total proteome of three methyltransferase gene knockouts strains of Escherichia coli: ΔrsmF (JW5301), ΔrlmC (JW2756), ΔrlmE (JW3146) from Keio collection and wild type (WT). Proteins were assessed using IDA approach (i.e. Information Dependent Acquisition) and SWATH (Data-Independent Acquisition) on Sciex TripleTof 5600+ mass‐spectrometer with a NanoSpray III ion source (ABSciex, Canada) coupled to a NanoLC Ultra 2D+ nano‐HPLC system (Eksigent). Dataset covers 16 samples.
HostingRepository	PRIDE
AnnounceDate	2024-10-22
AnnouncementXML	Submission_2024-10-22_04:59:26.535.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Olga Bukato
SpeciesList	scientific name: Escherichia coli; NCBI TaxID: 562;
ModificationList	No PTMs are included in the dataset
Instrument	TripleTOF 5600

Dataset History

Revision	Datetime	Status	ChangeLog Entry
0	2020-01-17 06:02:09	ID requested
1	2020-01-21 02:00:21	announced
⏵ 2	2024-10-22 04:59:26	announced	2024-10-22: Updated project metadata.

Publication List

Dataset with its publication pending

Dataset with its publication pending

Keyword List

submitter keyword: LC-MSMS,Escherichia coli, IDA

submitter keyword: LC-MSMS,Escherichia coli, IDA

Contact List

Petr Sergiev
contact affiliation	Lomonosov Moscow State University, Moscow, Russia
contact email	petya@belozersky.msu.ru
lab head
Olga Bukato
contact affiliation	Federal Research and Clinical Center of Physical-Chemical Medicine of Federal Medical Biological Agency
contact email	olya.bukato@gmail.com
dataset submitter

Full Dataset Link List

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2020/01/PXD017171
PRIDE project URI

Dataset FTP location
NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2020/01/PXD017171

PRIDE project URI

Repository Record List

[ + ]