<<< Full experiment listing

PXD017067

PXD017067 is an original dataset announced via ProteomeXchange.

Dataset Summary
TitleThe dynamic surface proteome of allergenic fungal conidia
DescriptionFungal spores and hyphal fragments are major contributors to the allergic response in the human lung. In this study, using a trypsin-shaving-proteomics approach, we identified the surface-exposed and secreted/shed proteins of Aspergillus fumigatus conidia and investigated the dynamics of the surface proteome under different conditions, including temperature variation and germination. We find that the surface proteome of resting A. fumigatus conidia is quite dynamic, as evidenced by drastically different surface proteomes under different growth conditions. We further investigated two observed A. fumigatus surface proteins, ScwA and CweA; ScwA is specific to the Aspergillus genus and only expressed on conidia grown at higher temperatures, whereas CweA is found throughout the Aspergillus and Penicillium genera. We extended our analysis of the surface proteome of A. fumigatus to other allergy-inducing pathogens; Alternaria alternata, Penicillium rubens, and Cladosporium herbarum, and performed comparative proteomics on resting and swollen conidia, as well as secreted proteins from germinating conidia. In this dataset, we detected 125 protein orthologs in the extracellular region of all four organisms, and 42 nonorthologous proteins produced by A. fumigatus, which may be useful in the development of future diagnostics. This study highlights the dynamic nature of the conidial surface and implicates growth conditions in the antigenicity of fungal conidia.
HostingRepositoryPRIDE
AnnounceDate2024-10-22
AnnouncementXMLSubmission_2024-10-22_05:00:31.749.xml
DigitalObjectIdentifier
ReviewLevelPeer-reviewed dataset
DatasetOriginOriginal dataset
RepositorySupportUnsupported dataset by repository
PrimarySubmitterThomas Krüger
SpeciesList scientific name: Penicillium rubens; NCBI TaxID: 1108849; scientific name: Cladosporium herbarum; NCBI TaxID: 29918; scientific name: Neosartorya fumigata (Aspergillus fumigatus); NCBI TaxID: 746128; scientific name: Alternaria alternata; NCBI TaxID: 5599;
ModificationListmonohydroxylated residue; iodoacetamide derivatized residue
InstrumentQ Exactive
Dataset History
RevisionDatetimeStatusChangeLog Entry
02020-01-10 06:44:35ID requested
12020-01-24 09:42:23announced
22024-10-22 05:00:32announced2024-10-22: Updated project metadata.
Publication List
Dataset with its publication pending
Keyword List
curator keyword: Biological, Biomedical
submitter keyword: hyphae, Aspergillus fumigatus, Penicillium rubens, germling, Cladosporium herbarum,surfome, Alternaria alternata, secretome, LC-MS/MS
Contact List
Axel A. Brakhage
contact affiliationDepartment of Molecular and Applied Microbiology Leibniz Institute for Natural Product Research and Infection Biology - Hans Knöll Institute
contact emailaxel.brakhage@leibniz-hki.de
lab head
Thomas Krüger
contact affiliationLeibniz Institute for Natural Product Research and Infection Biology - Hans Knöll Institute
contact emailthomas.krueger@leibniz-hki.de
dataset submitter
Full Dataset Link List
Dataset FTP location
NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2020/01/PXD017067
PRIDE project URI
Repository Record List
[ + ]