PXD016558
PXD016558 is an original dataset announced via ProteomeXchange.
Dataset Summary
Title | EGFR inhibition triggers an adaptive response by co-opting antiviral signaling pathways in lung cancer |
Description | HCC827 cells were treated with 0.1 microM erlotinib for 0, 2, 6, and 24 hours. Cell lysates were immunoprecipitated with TBK1 antibody. Antibody enriched protein samples were run on SDS-PAGE gels and submitted to UT Southwestern Proteomics Core Facility for Mass Spectrometry. Protein gel pieces were digested overnight with trypsin (Pierce) following reduction and alkylation with DTT and iodoacetamide (Sigma Aldrich. The samples then underwent solid-phase extraction cleanup with Oasis HLB microelution plates (Waters) and the resulting samples were analyzed by LC-MS-MS, using an Orbitrap Fusion Lumos mass spectrometer (Thermo Electron) coupled to an Ultimate 3000 RSLC-Nano liquid chromatography systems (Dionex). |
HostingRepository | MassIVE |
AnnounceDate | 2019-12-03 |
AnnouncementXML | Submission_2019-12-03_12:49:59.xml |
DigitalObjectIdentifier | |
ReviewLevel | Non peer-reviewed dataset |
DatasetOrigin | Original dataset |
RepositorySupport | Unsupported dataset by repository |
PrimarySubmitter | Mohammad |
SpeciesList | scientific name: Homo sapiens; common name: human; NCBI TaxID: 9606; |
ModificationList | No PTMs are included in the dataset |
Instrument | Orbitrap Fusion Lumos |
Dataset History
Revision | Datetime | Status | ChangeLog Entry |
---|---|---|---|
0 | 2019-12-03 12:45:30 | ID requested | |
⏵ 1 | 2019-12-03 12:50:00 | announced |
Publication List
no publication |
Keyword List
submitter keyword: EGFR inhibition, therapeutic resistance, Type I interferons, TBK1, IRF3,RIG-I, lung cancer, NSCLC, erlotinib, EGFR wild type, Anifrolumab |
Contact List
Amyn A. Habib | |
---|---|
contact affiliation | University of Texas Southwestern Medical Center, North Texas VA Healthcare System, Mail Code 151, 4500 South Lancaster Road, Dallas TX 75216 |
contact email | Amyn.Habib@UTSouthwestern.edu |
lab head | |
Mohammad | |
contact affiliation | UT Southwestern Medical center |
contact email | mohammad.goodarzi@utsouthwestern.edu |
dataset submitter |
Full Dataset Link List
MassIVE dataset URI |
Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://massive.ucsd.edu/MSV000084654/ |