PXD015525

PXD015525 is an original dataset announced via ProteomeXchange.

Title	HipBA toxin-antitoxin system Caulobacter crescentus.
Description	Antibiotic persistence is a transient phenotypic state during which a bacterium can withstand otherwise lethal antibiotic exposure or environmental stresses. In Escherichia coli, persistence is promoted by the HipBA toxin-antitoxin system. The HipA toxin functions as a serine/threonine kinase that inhibits cell growth, while the HipB antitoxin neutralizes the toxin. E. coli HipA inactivates the glutamyl-tRNA synthetase GltX, which inhibits translation and triggers the highly conserved stringent response. Although hipBA operons are widespread in bacterial genomes, it is unknown if this mechanism is conserved in other species. Here we describe the functions of three hipBA modules in the alpha-proteobacterium Caulobacter crescentus. The HipA toxins have different effects on growth and macromolecular syntheses, and they phosphorylate distinct substrates. HipA1 and HipA2 contribute to antibiotic persistence during stationary phase by phosphorylating the aminoacyl-tRNA synthetases GltX and TrpS. The stringent response regulator SpoT is required for HipA-mediated antibiotic persistence, but persister cells can form in the absence of all hipBA operons or spoT, indicating that multiple pathways lead to persister cell formation in C. crescentus.
HostingRepository	PRIDE
AnnounceDate	2024-10-22
AnnouncementXML	Submission_2024-10-22_04:58:51.624.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Celine Henry
SpeciesList	scientific name: Caulobacter crescentus (strain NA1000 / CB15N); NCBI TaxID: 565050;
ModificationList	phosphorylated residue
Instrument	Q Exactive

Revision	Datetime	Status	ChangeLog Entry
0	2019-09-20 06:15:22	ID requested
1	2020-02-06 01:53:24	announced
⏵ 2	2024-10-22 04:58:52	announced	2024-10-22: Updated project metadata.

Dataset with its publication pending

submitter keyword: Caulobacter crescentus, phosphoproteome

Celine Henry
contact affiliation	INRA, Micalis institute
contact email	celine.henry@inra.fr
lab head
Celine Henry
contact affiliation	INRAE
contact email	celine.henry@inra.fr
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD015525
     1. Label: PRIDE project
     2. Name: HipBA toxin-antitoxin system Caulobacter crescentus.