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PXD015488

PXD015488 is an original dataset announced via ProteomeXchange.

Dataset Summary
TitleGcn5 and Esa1 function as histone crotonyltransferases to regulate crotonylation-dependent transcription
DescriptionHistone post-translational modifications (PTMs) are critical for processes such as transcription. The more notable among these are the non-acetyl histone lysine acylation modifications such as crotonylation, butyrylation and succinylation. However, the biological relevance of these PTMs is not fully understood because their regulation is largely unknown. Here, we set out to investigate whether the main histone acetyltransferases in budding yeast, Gcn5 and Esa1, possess crotonyltransferase activity. In vitro studies revealed that the Gcn5-Ada2-Ada3 (ADA) and Esa1-Yng2-Epl1 (Piccolo NuA4) histone acetyltransferase complexes have the capacity to crotonylate histones. Mass spectrometry analysis revealed that ADA and Piccolo NuA4 crotonylate lysines in the N-terminal tails of histone H3 and H4, respectively. Functionally, we show that crotonylation selectively affects gene transcription in vivo in a manner dependent on Gcn5 and Esa1. Thus, we identify the Gcn5- and Esa1-containing ADA and Piccolo NuA4 complexes as bona fide crotonyltransferases that promote crotonylation-dependent transcription.
HostingRepositoryPRIDE
AnnounceDate2024-10-22
AnnouncementXMLSubmission_2024-10-22_04:57:48.820.xml
DigitalObjectIdentifier
ReviewLevelPeer-reviewed dataset
DatasetOriginOriginal dataset
RepositorySupportUnsupported dataset by repository
PrimarySubmitterG Janssen
SpeciesList scientific name: Xenopus laevis (African clawed frog); NCBI TaxID: 8355; scientific name: Saccharomyces cerevisiae (Baker's yeast); NCBI TaxID: 4932;
ModificationListN6-crotonyl-L-lysine; acetylated residue
InstrumentOrbitrap Fusion Lumos; Q Exactive
Dataset History
RevisionDatetimeStatusChangeLog Entry
02019-09-17 07:58:46ID requested
12019-11-06 07:57:53announced
22024-10-22 04:57:49announced2024-10-22: Updated project metadata.
Publication List
Dataset with its publication pending
Keyword List
submitter keyword: Gcn5-Ada2-Ada3 (ADA) complex, histone modification, crotonylation, gene transcription,epigenetics, Esa1- Yng2-Epl1 (Piccolo NuA4) complex
Contact List
P.A. van Veelen
contact affiliationDr. P.A. van Veelen LUMC Centre for Proteomics and Metabolomics Head Proteomics group
contact emailP.A.van_Veelen@lumc.nl
lab head
G Janssen
contact affiliationLeiden University Medical Center
contact emailg.m.c.janssen@lumc.nl
dataset submitter
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Dataset FTP location
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