PXD015364 is an
original dataset announced via ProteomeXchange.
Dataset Summary
Title | A label-free shotgun proteomics analysis of macadamia nut |
Description | In this study, we present a first proteomic overview of macadamia nut using a label-free shotgun proteomic approach and prediction of their proteins with potential allergenic activity and cross-reactivity via an in silico analysis. The defense response proteins were the most abundant group of proteins and accounted for 24% of the total abundance. The vicilin-like macadamia antimicrobial peptides 2-3 (MiAMP2) was the most abundant protein. The cascading of amino acid and carbohydrate metabolic pathways in macadamia nut were constructed and proposed for the first time. The in silico analysis revealed seed storage proteins (conglutins and vicilins), rubber elongation factor proteins, phosphate binding proteins, and detoxifying methylglyoxalases among others as proteins with allergenic and cross-reactive potential. The label-free shotgun proteomics study revealed valuable insight into the genetic and biological makeup of macadamia nut and potential proteins with predictive allergenic activity and cross-reactivity. Integration of genomics with the proteomic data will be indispensable for future molecular characterization of macadamia including allergens. |
HostingRepository | PRIDE |
AnnounceDate | 2023-11-14 |
AnnouncementXML | Submission_2023-11-14_06:55:26.266.xml |
DigitalObjectIdentifier | |
ReviewLevel | Peer-reviewed dataset |
DatasetOrigin | Original dataset |
RepositorySupport | Unsupported dataset by repository |
PrimarySubmitter | sridevi muralidharan |
SpeciesList | scientific name: Macadamia; NCBI TaxID: 4329; |
ModificationList | monohydroxylated residue; iodoacetamide derivatized residue |
Instrument | LTQ FT |
Dataset History
Revision | Datetime | Status | ChangeLog Entry |
0 | 2019-09-09 03:21:52 | ID requested | |
1 | 2020-01-13 04:44:03 | announced | |
2 | 2020-01-15 06:12:11 | announced | 2020-01-15: Updated publication reference for DOI(s): 10.1016/j.foodres.2019.108838. |
⏵ 3 | 2023-11-14 06:55:26 | announced | 2023-11-14: Updated project metadata. |
Publication List
Dataset with its publication pending |
Keyword List
submitter keyword: Macadamia nut |
label-free shotgun proteomics |
in silico analysis |
allergenicity |
cross-reactivity |
Contact List
N Alice Lee |
contact affiliation | ARC Training Centre for Advanced Technologies in Food Manufacture, School of Chemical Engineering, UNSW SYDNEY, NSW 2052, AUSTRALIA |
contact email | alice.lee@unsw.edu.au |
lab head | |
sridevi muralidharan |
contact affiliation | UNSW |
contact email | sridevi03@gmail.com |
dataset submitter | |
Full Dataset Link List
Dataset FTP location
NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2020/01/PXD015364 |
PRIDE project URI |
Repository Record List
[ + ]
[ - ]
- PRIDE
- PXD015364
- Label: PRIDE project
- Name: A label-free shotgun proteomics analysis of macadamia nut