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PXD014988

PXD014988 is an original dataset announced via ProteomeXchange.

Dataset Summary
TitleLong non-coding RNA HOTTIP promotes CCL3 expression and induces cartilage degradation by sponging miR-455-3p
DescriptionLong non-coding RNAs (lncRNAs) play pivotal roles in diseases such as osteoarthritis (OA). However, knowledge of the biological roles of lncRNAs is limited in OA. We aimed to explore the biological function and molecular mechanism of HOTTIP in chondrogenesis and cartilage degradation. We used the human mesenchymal stem cell (MSC) model of chondrogenesis, in parallel with, tissue biopsies from normal and OA cartilage to detect HOTTIP, CCL3, and miR-455-3p expression in vitro. Biological interactions between HOTTIP and miR-455-3p were determined by RNA silencing and overexpression in vitro. We evaluated the effect of HOTTIP on chondrogenesis and degeneration, and its regulation of miR-455-3p via competing endogenous RNA (ceRNA). Our in vitro ceRNA findings were further confirmed within animal models in vivo. Mechanisms of ceRNAs were determined by bioinformatic analysis, a luciferase reporter system, RNA pull-down, and RNA immunoprecipitation (RIP) assays. We found reduced miR-455-3p expression and significantly upregulated lncRNA HOTTIP and CCL3 expression in OA cartilage tissues and chondrocytes. The expression of HOTTIP and CCL3 was increased in chondrocytes treated with interleukin-1β (IL-1β) in vitro. Knockdown of HOTTIP promoted cartilage-specific gene expression and suppressed CCL3. Conversely, HOTTIP overexpression reduced cartilage-specific genes and increased CCL3. Notably, HOTTIP negatively regulated miR-455-3p and increased CCL3 levels in human primary chondrocytes. Mechanistic investigations indicated that HOTTIP functioned as ceRNA for miR-455-3p enhanced CCL3 expression. Taken together, the ceRNA regulatory network of HOTTIP/miR-455-3p/CCL3 plays a critical role in OA pathogenesis and suggests HOTTIP is a potential target in OA therapy.
HostingRepositoryPRIDE
AnnounceDate2019-08-12
AnnouncementXMLSubmission_2019-08-12_06:24:05.xml
DigitalObjectIdentifier
ReviewLevelPeer-reviewed dataset
DatasetOriginOriginal dataset
RepositorySupportUnsupported dataset by repository
PrimarySubmitterguping mao
SpeciesList scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationListNo PTMs are included in the dataset
InstrumentLCQ Classic
Dataset History
RevisionDatetimeStatusChangeLog Entry
02019-08-12 02:22:45ID requested
12019-08-12 06:24:06announced
Publication List
Dataset with its publication pending
Keyword List
ProteomeXchange project tag: Skeletal Muscle Proteome (B/D-HPP)
submitter keyword: HUMAN-CHONDROCYTE-MS
Contact List
Guping Mao
contact affiliationthe first affiliated hospital, sun yat-sen university
contact emailguping0913@163.com
lab head
guping mao
contact affiliationthe first affiliated hospital,sun yat-sen university
contact emailGUPING0913@163.COM
dataset submitter
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