PXD014983

PXD014983 is an original dataset announced via ProteomeXchange.

Title	A technology to capture DNA binding proteins based on biolistic transformation
Description	Interactions between DNA and proteins are critical to almost all cellular functions, and the identification of proteins that bind to a DNA sequence of interest (gene-centered approach) is a widely studied area. However, a limited number of gene-centered methods have been developed. In the present study, we built a protein capture technology based on biolistic transformation (PCaT) to capture proteins bound by a target DNA. A promoter sequence or a DNA fragment was labeled with biotin and introduced into the host plants using biolistic transformation. The DNA and its bound proteins were cross-linked using formaldehyde in the transformed plants, and the labeled DNAs were captured using streptavidin magnetic beads, which enabled harvesting of the DNA-bound proteins. After mass spectrum analysis, the proteins associated with the target DNA were identified. Using this method, we identified the upstream regulators of a WRKY gene from Populus davidiana×P. bolleana. The reliability of the identification of the upstream regulators of WRKY was confirmed using chromatin immunoprecipitation and electrophoretic mobility shift assays. Taken together, the results showed PCaT provides a novel DNA and protein interactome discovery strategy. In addition, based on PCaT, a quick procedure to determine the upstream regulators of a gene of interest was proposed.
HostingRepository	iProX
AnnounceDate	2019-08-12
AnnouncementXML	Submission_2019-08-12_02:15:11.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Xin Zhang
SpeciesList	scientific name: Populus davidiana x Populus alba var. pyramidalis; NCBI TaxID: 1550601;
ModificationList	natural residue
Instrument	Q Exactive

Revision	Datetime	Status	ChangeLog Entry
0	2019-08-12 02:13:29	ID requested
⏵ 1	2019-08-12 02:15:12	announced

Dataset with its publication pending

submitter keyword: Biolistic transformation, biotin, DNA binding proteins, gene-centered approach, mass spectrum, streptavidin magnetic beads

Chuanping Yang
contact affiliation	State Key Laboratory of Tree Genetics and Breeding
contact email	goodxin2016@163.com
lab head
Xin Zhang
contact affiliation	State Key Laboratory of Tree Genetics and Breeding
contact email	goodxin2016@nefu.edu.cn
dataset submitter

iProX dataset URI