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PXD014694

PXD014694 is an original dataset announced via ProteomeXchange.

Dataset Summary
TitleProteomic analysis of extracellular vesicles derived from platelet concentrates treated with MirasolĀ® identifies biomarkers of platelet storage lesion
DescriptionIn blood banks, platelets are stored until 7 days after a pathogen reduction technology (PRT) treatment, MirasolĀ® (vitamin B2 plus UVB light) in the present case. The storage time under these conditions may have an impact on platelets and their releasate leading to potential adverse reactions following transfusion to patients. The aim of this study was to analyze the proteome of extracellular vesicles generated by platelets at different storage days (2 and 7) to gain deeper information on the platelet concentrates state at those moments. EVs were isolated by a centrifugation-based approach and characterized by dynamic light scattering and transmission electron microscopy. Proteomic analysis was by LC-MS/MS and quantification by SWATH. In this way, 151 proteins were found up-regulated at day 7 of storage. This group includes CCL5 and Platelet factor 4, chemokines with power to attract neutrophils and monocytes, which could generate transfusion adverse reactions. In addition, other glycoproteins and platelet activation markers were also found elevated at day 7. Proteins related to glycolysis and lactate production were found altered with high fold changes, showing a deregulation of platelet metabolism at day 7. The obtained results provide novel information about possible effects of platelet-derived EVs on transfusion adverse reactions.
HostingRepositoryPRIDE
AnnounceDate2023-11-14
AnnouncementXMLSubmission_2023-11-14_08:50:19.840.xml
DigitalObjectIdentifier
ReviewLevelPeer-reviewed dataset
DatasetOriginOriginal dataset
RepositorySupportUnsupported dataset by repository
PrimarySubmitterSusana Bravo
SpeciesList scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationListNo PTMs are included in the dataset
InstrumentTripleTOF 5600
Dataset History
RevisionDatetimeStatusChangeLog Entry
02019-07-22 06:46:11ID requested
12022-09-30 01:02:20announced
22023-11-14 08:50:20announced2023-11-14: Updated project metadata.
Publication List
Dataset with its publication pending
Keyword List
submitter keyword: Extracellular vesicles
platelet concentrates
transfusion medicine
SWATH
Contact List
Susana B. Bravo
contact affiliationFIDIS
contact emailsbbravo@gmail.com
lab head
Susana Bravo
contact affiliationFIDIS
contact emailsbbravo@gmail.com
dataset submitter
Full Dataset Link List
Dataset FTP location
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PRIDE project URI
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