PXD014694 is an
original dataset announced via ProteomeXchange.
Dataset Summary
Title | Proteomic analysis of extracellular vesicles derived from platelet concentrates treated with MirasolĀ® identifies biomarkers of platelet storage lesion |
Description | In blood banks, platelets are stored until 7 days after a pathogen reduction technology (PRT) treatment, MirasolĀ® (vitamin B2 plus UVB light) in the present case. The storage time under these conditions may have an impact on platelets and their releasate leading to potential adverse reactions following transfusion to patients. The aim of this study was to analyze the proteome of extracellular vesicles generated by platelets at different storage days (2 and 7) to gain deeper information on the platelet concentrates state at those moments. EVs were isolated by a centrifugation-based approach and characterized by dynamic light scattering and transmission electron microscopy. Proteomic analysis was by LC-MS/MS and quantification by SWATH. In this way, 151 proteins were found up-regulated at day 7 of storage. This group includes CCL5 and Platelet factor 4, chemokines with power to attract neutrophils and monocytes, which could generate transfusion adverse reactions. In addition, other glycoproteins and platelet activation markers were also found elevated at day 7. Proteins related to glycolysis and lactate production were found altered with high fold changes, showing a deregulation of platelet metabolism at day 7. The obtained results provide novel information about possible effects of platelet-derived EVs on transfusion adverse reactions. |
HostingRepository | PRIDE |
AnnounceDate | 2023-11-14 |
AnnouncementXML | Submission_2023-11-14_08:50:19.840.xml |
DigitalObjectIdentifier | |
ReviewLevel | Peer-reviewed dataset |
DatasetOrigin | Original dataset |
RepositorySupport | Unsupported dataset by repository |
PrimarySubmitter | Susana Bravo |
SpeciesList | scientific name: Homo sapiens (Human); NCBI TaxID: 9606; |
ModificationList | No PTMs are included in the dataset |
Instrument | TripleTOF 5600 |
Dataset History
Revision | Datetime | Status | ChangeLog Entry |
0 | 2019-07-22 06:46:11 | ID requested | |
1 | 2022-09-30 01:02:20 | announced | |
⏵ 2 | 2023-11-14 08:50:20 | announced | 2023-11-14: Updated project metadata. |
Publication List
Dataset with its publication pending |
Keyword List
submitter keyword: Extracellular vesicles |
platelet concentrates |
transfusion medicine |
SWATH |
Contact List
Susana B. Bravo |
contact affiliation | FIDIS |
contact email | sbbravo@gmail.com |
lab head | |
Susana Bravo |
contact affiliation | FIDIS |
contact email | sbbravo@gmail.com |
dataset submitter | |
Full Dataset Link List
Dataset FTP location
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PRIDE project URI |
Repository Record List
[ + ]
[ - ]
- PRIDE
- PXD014694
- Label: PRIDE project
- Name: Proteomic analysis of extracellular vesicles derived from platelet concentrates treated with MirasolĀ® identifies biomarkers of platelet storage lesion