PXD014280
PXD014280 is an original dataset announced via ProteomeXchange.
Dataset Summary
Title | Profiling the surfaceome identifies therapeutic targets for cells with hyperactive mTORC1 signaling |
Description | Aberrantly high mTORC1 signaling is a known driver of many cancers and human disorders, yet pharmacological inhibition of mTORC1 rarely confers durable clinical responses. To explore alternative therapeutic strategies, herein we conducted a proteomics survey to identify cell surface proteins upregulated by mTORC1. A comparison of the surfaceome from Tsc1-/- versus Tsc1+/+ mouse embryonic fibroblasts revealed 59 proteins predicted to be significantly overexpressed in Tsc1-/- cells. Further validation of the data in multiple mouse and human cell lines showed that mTORC1 signaling most dramatically induced the expression of the proteases neprilysin (NEP/CD10) and aminopeptidase N (APN/CD13). Functional studies showed that constitutive mTORC1 signaling sensitized cells to genetic ablation of NEP and APN, as well as the biochemical inhibition of APN. In summary, these data show that mTORC1 signaling plays a significant role in the constitution of the surfaceome, which in turn may present novel therapeutic strategies. |
HostingRepository | MassIVE |
AnnounceDate | 2020-01-15 |
AnnouncementXML | Submission_2020-01-15_11:28:24.xml |
DigitalObjectIdentifier | |
ReviewLevel | Non peer-reviewed dataset |
DatasetOrigin | Original dataset |
RepositorySupport | Supported dataset by repository |
PrimarySubmitter | Kevin Leung |
SpeciesList | scientific name: Mus musculus; common name: house mouse; NCBI TaxID: 10090; |
ModificationList | S-carboxamidomethyl-L-cysteine; deglycosylated asparagine; 6x(13)C,4x(15)N labeled L-arginine; 6x(13)C,2x(15)N labeled L-lysine |
Instrument | Q Exactive |
Dataset History
Revision | Datetime | Status | ChangeLog Entry |
---|---|---|---|
0 | 2019-06-17 19:16:56 | ID requested | |
⏵ 1 | 2020-01-15 11:28:25 | announced |
Publication List
no publication |
Keyword List
submitter keyword: SILAC, TSC, mTORC1 singaling, Drug target |
Contact List
Kevin K Leung | |
---|---|
contact affiliation | UCSF |
contact email | kevin.leung@ucsf.edu |
lab head | |
Kevin Leung | |
contact affiliation | UCSF |
contact email | kevin.leung@ucsf.edu |
dataset submitter |
Full Dataset Link List
MassIVE dataset URI |
Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://massive.ucsd.edu/MSV000083988/ |