PXD013999

PXD013999 is an original dataset announced via ProteomeXchange.

Title	Multiple C2 domains and Transmembrane region Proteins (MCTPs) tether membranes at plasmodesmata
Description	In eukaryotes, membrane contact sites (MCS) allow direct communication between organelles. Plants have evolved unique MCS, the plasmodesmata intercellular pores, which combine organelle tethering with regulation of cell-to-cell signalling, the molecular mechanisms of which remains unknown. Here, we identify Multiple C2 domains and Transmembrane region Proteins (MCTPs) as tethers that link the endoplasmic reticulum (ER) to the plasma membrane (PM) within plasmodesmata. We report that MCTPs, including MCTP3 and 4 recently identified as modulators of SHOOTMERISTEMLESS trafficking, are ER-anchored proteins that cluster at plasmodesmata. MCTPs insert into the ER via their transmembrane region whilst their C2 domains dock to the PM through interaction with anionic phospholipids. A mctp3/4 loss-of function mutant induces plant developmental defects while MCTP4 expression in a yeast .tether mutant partially restores ER-PM tethering. Our data suggest that MCTPs are unique membrane tethers controlling both ER-PM contacts and cell-cell signalling.
HostingRepository	PRIDE
AnnounceDate	2019-07-08
AnnouncementXML	Submission_2019-07-12_03:03:31.xml
DigitalObjectIdentifier	https://dx.doi.org/10.6019/PXD013999
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Supported dataset by repository
PrimarySubmitter	Max Kraner
SpeciesList	scientific name: Arabidopsis thaliana (Mouse-ear cress); NCBI TaxID: 3702;
ModificationList	Oxidation; Carbamidomethyl
Instrument	Orbitrap Fusion

Revision	Datetime	Status	ChangeLog Entry
0	2019-05-23 23:50:59	ID requested
1	2019-07-08 09:11:07	announced
⏵ 2	2019-07-12 03:03:33	announced	Updated publication reference for PubMed record(s): 31286648.

Brault ML, Petit JD, Immel F, Nicolas WJ, Glavier M, Brocard L, Gaston A, Fouch, é M, Hawkins TJ, Crowet JM, Grison MS, Germain V, Rocher M, Kraner M, Alva V, Claverol S, Paterlini A, Helariutta Y, Deleu M, Lins L, Tilsner J, Bayer EM, Multiple C2 domains and transmembrane region proteins (MCTPs) tether membranes at plasmodesmata. EMBO Rep, 20(8):e47182(2019) [pubmed]

curator keyword: Biological

submitter keyword: plasmodesmata, LC-MS

Max Edmund Kraner
contact affiliation	LS Biochemistry, Biology, Friedrich-Alexander-University
contact email	max.kraner@fau.de
lab head
Max Kraner
contact affiliation	FAU Erlangen
contact email	max.kraner@fau.de
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD013999
     1. Label: PRIDE project
     2. Name: Multiple C2 domains and Transmembrane region Proteins (MCTPs) tether membranes at plasmodesmata