PXD013961

PXD013961 is an original dataset announced via ProteomeXchange.

Title	Proteome of L-carnitine-grown Eubacterium limosum ATCC 8486
Description	Eubacterium limosum ATCC 8486 makes acetate and butyrate from various substrates and is found in the human intestine. The proteome of L-carnitine-grown Eubacterium limosum was obtained in order to identify enzymes required for growth on L-carnitine, in particular to identify components that are unique to growth on L-carnitine in comparison to other substrates for acetogenesis, such as lactic acid. L-carnitine and derviatives are converted to trimethylamine (TMA) by certain members of the gut microbiome, metabolism of TMA is now tied to progression of cardiovascular disease. Demethylation of carnitine is observed during growth of Eubacterium limosum on this substrate, and does not produce TMA. Carnitine demethylation by organisms like Eubacterium limosum could lessen TMA production in the gut, thereby lessening the propensity towards atherorsclerosis caused by metabolism of TMA in the body. The carnitine proteome led to the description of a carnitine:tetrahydrofolate methyltransferase system. The key carnitine demethylating enzyme is a member of the widespread TMA methyltransferase protein superfamily.
HostingRepository	PRIDE
AnnounceDate	2020-06-19
AnnouncementXML	Submission_2020-06-18_22:10:12.xml
DigitalObjectIdentifier	https://dx.doi.org/10.6019/PXD013961
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Supported dataset by repository
PrimarySubmitter	Joseph Krzycki
SpeciesList	scientific name: Eubacterium limosum; NCBI TaxID: 1736;
ModificationList	Deamidated; Oxidation; Carbamidomethyl
Instrument	Orbitrap Fusion

Revision	Datetime	Status	ChangeLog Entry
0	2019-05-22 01:49:04	ID requested
⏵ 1	2020-06-18 22:10:13	announced

Dataset with its publication pending

submitter keyword: Acetogenic bacteria, human gut isolate, carnitine demethylation

Joseph Adrian Krzycki
contact affiliation	Department of Microbiology The Ohio State University Columbus OH 43210
contact email	Krzycki.1@osu.edu
lab head
Joseph Krzycki
contact affiliation	The Ohio State University
contact email	Krzycki.1@osu.edu
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD013961
     1. Label: PRIDE project
     2. Name: Proteome of L-carnitine-grown Eubacterium limosum ATCC 8486