PXD013272
PXD013272 is an original dataset announced via ProteomeXchange.
Dataset Summary
| Title | Paenibacillus larvae exoproteins of ERIC I-IV genotypes |
| Description | The following strains of Paenibacillus larvae classified as ERIC I-IV genotypes (pl1-pl4) were used in the study: ERIC I - DSM-7030; ERIC II - DSM-25430; ERIC III - LMG-16252; and ERIC IV - DSM-3615. The 7 biological replicates (a-g) of the exoprotein fractions prepared from each ERIC genotype were used in the nanoLC-MS/MS analysis. All 28 samples were consecutively analyzed using an OrbitrapTM FusionTM Tribrid q-OT-IT mass spectrometer. To improve the protein identification and easy exclude contaminants from the medium, we considered the proteins that could originate from the yeast extract and Mueller-Hinton broth (contains beef fusion and casein peptone-acidic hydrolysate) in analyzing the MS data. Thus, we included the sequences related to Saccharomyces cerevisiae and Bos taurus, respectively, to the list of contaminants in MaxQuant. The contaminants.fasta file is provided. Futhermore, we provide the database (uniprot-paenibacillus+larvae.fasta) used for the identification of P. larvae proteins created of different strains of P. larvae. The sequences were downloaded of UniProt on 12.03.2019. Finally, the compressed (zipped) folder "combined" is provided. The data were analyzed in MaxQuant v1.6.3.4. |
| HostingRepository | MassIVE |
| AnnounceDate | 2019-04-11 |
| AnnouncementXML | Submission_2019-04-11_07:20:08.xml |
| DigitalObjectIdentifier | |
| ReviewLevel | Non peer-reviewed dataset |
| DatasetOrigin | Original dataset |
| RepositorySupport | Unsupported dataset by repository |
| PrimarySubmitter | Tomas Erban |
| SpeciesList | scientific name: Paenibacillus larvae; NCBI TaxID: 1464; |
| ModificationList | Methylthio; unknown modification: Oxidation (M); unknown modification: Acetyl (Protein N-term) |
| Instrument | Orbitrap Fusion |
Dataset History
| Revision | Datetime | Status | ChangeLog Entry |
|---|---|---|---|
| 0 | 2019-03-27 15:52:52 | ID requested | |
| ⏵ 1 | 2019-04-11 07:30:49 | announced |
Publication List
| no publication |
Keyword List
| submitter keyword: Paenibacillus larvae, Paenibacillus larvae subsp. larvae, Paenibacillus larvae subsp. pulvifaciens, American foulbrood, pathogen, honey bee, Apis mellifera , exoproteins, virulence factors |
Contact List
| Tomas Erban | |
|---|---|
| contact affiliation | Crop Research Institute |
| contact email | arachnid@centrum.cz |
| lab head | |
| Tomas Erban | |
| contact affiliation | Crop Research Institute |
| contact email | arachnid@centrum.cz |
| dataset submitter | |
Full Dataset Link List
| MassIVE dataset URI |
| Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://massive.ucsd.edu/MSV000083636 |
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